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dc.contributor.author
Biolatti, Luisa Vanesa
dc.contributor.author
Negrin, Lara Maria
dc.contributor.author
Bellora, Nicolás
dc.contributor.author
Ibañez, Irene Laura
dc.date.available
2022-05-05T19:11:10Z
dc.date.issued
2021-01
dc.identifier.citation
Biolatti, Luisa Vanesa; Negrin, Lara Maria; Bellora, Nicolás; Ibañez, Irene Laura; High-throughput meta-analysis and validation of differentially expressed genes as potential biomarkers of ionizing radiation-response; Elsevier Ireland; Radiotherapy And Oncology; 154; 1-2021; 21-28
dc.identifier.issn
0167-8140
dc.identifier.uri
http://hdl.handle.net/11336/156683
dc.description.abstract
Background and purpose: The high-throughput analysis of gene expression in ionizing radiation (IR)-exposed human peripheral white blood cells (WBC) has emerged as a novel method for biodosimetry markers detection. We aimed to detect IR-exposure differential expressed genes (DEGs) as potential predictive biomarkers for biodosimetry and radioinduced-response. Materials and methods: We performed a meta-analysis of raw data from public microarrays of ex vivo low linear energy transfer-irradiated human peripheral WBC. Functional enrichment and transcription factors (TF) detection from resulting DEGs were assessed. Six selected DEGs among studies were validated by qRT-PCR on mRNA from human peripheral blood samples from nine healthy human donors 24 h after ex vivo X-rays-irradiation. Results: We identified 275 DEGs after IR-exposure (parameters: |lfc| ≥ 0.7, q value <0.05), enriched in processes such as regulation after IR-exposure, DNA damage checkpoint, signal transduction by p53 and mitotic cell cycle checkpoint. Among these DEGs, DRAM1, NUDT15, PCNA, PLK2 and TIGAR were selected for qRT-PCR validation. Their expression levels significantly increased at 1–4 Gy respect to non-irradiated controls. Particularly, PCNA increased dose dependently. Curiously, TCF4 (Entrez Gene: 6925), detected as overrepresented TF in the radioinduced DEGs set, significantly decreased post-irradiation. Conclusion: These six DEGs show potential to be proposed as candidates for IR-exposure biomarkers, considering their observed molecular radioinduced-response. Among them, TCF4, bioinformatically detected, was validated herein as an IR-responsive gene.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Ireland
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BIODOSIMETRY
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BIOINFORMATICS
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HUMAN PERIPHERAL WHITE BLOOD CELLS
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MICROARRAYS
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X RAYS IRRADIATION
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
High-throughput meta-analysis and validation of differentially expressed genes as potential biomarkers of ionizing radiation-response
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-12-04T19:56:30Z
dc.journal.volume
154
dc.journal.pagination
21-28
dc.journal.pais
Irlanda
dc.journal.ciudad
Shannon
dc.description.fil
Fil: Biolatti, Luisa Vanesa. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Instituto de Tecnologías Nucleares para la Salud; Argentina
dc.description.fil
Fil: Negrin, Lara Maria. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Instituto de Tecnologías Nucleares para la Salud; Argentina
dc.description.fil
Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
dc.description.fil
Fil: Ibañez, Irene Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
dc.journal.title
Radiotherapy And Oncology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.thegreenjournal.com/article/S0167-8140(20)30782-9/fulltext
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.radonc.2020.09.010
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