Artículo
Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs
Pellegrino, Francisco Javier
; Risso, Analia Lorena
; Corrada, Yanina Alejandra
; Gambaro, Rocío Celeste; Seoane, Analia Isabel
Fecha de publicación:
06/2021
Editorial:
Wiley
Revista:
Veterinary Record Open
ISSN:
2052-6113
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Background: Fish oil (FO) supplementation as a source of omega 3 fatty acids is associated with beneficial effects on health. However, high unsaturated fatty acid content in thediet could result in increased lipid peroxidation and damage to proteins, lipids and DNA.We evaluated the effect of dietary FO supplementation on DNA damage in peripheralblood lymphocytes of dogs. Additionally, we determined the effect of FO supplementation on lipid peroxidation and lipid profile of these dogs. Methods: Healthy male dogs (n = 9) were randomly assigned to one of two diets during90 days: control (CG, n = 4), based on a commercial food, and FO (FOG, n = 5), thesame food supplemented with 1000 mg FO. Blood samples were collected on days −1,30, 60 and 90. DNA damage was assessed with the comet assay, and the damage indexwas obtained. Malondialdehyde (MDA) levels were determined as an indicator of lipidperoxidation. Lipid profile determination included serum triglyceride, cholesterol, lowdensity lipoprotein and high-density lipoprotein levels (HDL). Results: Damage index values (arbitrary units) were lower in FOG on day 30 (CG,13.7 ± 2.5; FOG, 6.5 ± 2.5), 60 (CG, 14.7 ± 2.5; FOG, 3.5 ± 2.5) and 90 (CG, 15.5 ±2.5; FOG, 3.0 ± 2.5) compared with CG (treatment × time interaction, p < 0.01). Serum MDA and HDL concentrations were lower in FOG compared with CG on day 60 and90 (treatment × time interaction, p < 0.05). Conclusion: These findings suggest that dietary FO supplementation did not induceDNA damage in peripheral blood lymphocytes of healthy dogs, but rather reduced it.
Palabras clave:
DOG
,
FISH OIL SUPPLEMENTATION
,
DNA DAMAGE
,
LYMPHOCYTES
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Articulos(IGEVET)
Articulos de INST.DE GENETICA VET ING FERNANDO NOEL DULOUT
Articulos de INST.DE GENETICA VET ING FERNANDO NOEL DULOUT
Citación
Pellegrino, Francisco Javier; Risso, Analia Lorena; Corrada, Yanina Alejandra; Gambaro, Rocío Celeste; Seoane, Analia Isabel; Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs; Wiley; Veterinary Record Open; 8; 1; 6-2021; 1-5
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