Artículo
Comparison of serological techniques for the diagnosis of equine infectious Anemia in an endemic area of Argentina
Espasandin, Ana Gabriela
; Cipolini, María F.; Forletti, Agustina
; Diaz, Silvina
; Soto, Javier; Martinez, Diana Elina; Storani, Carlos Alberto; Monzon, Nolly Maria
; Beltrame, Juan I.; Luchessi, Estéban; Soto, Pedro
Fecha de publicación:
05/2021
Editorial:
Elsevier Science
Revista:
Journal of Virological Methods
ISSN:
0166-0934
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Equine Infectious Anemia is a transmissible viral disease present worldwide, caused by an RNA virus. Viral transmission is mainly mechanical through blood or its products most frequently by blood-sucking arthropods and iatrogenesis as well. OIE recommends Coggins Test as the diagnostic method of choice. Some ELISA tests detect antibodies earlier than the Coggins test, but may produce false-positive results. Currently, new techniques for EIA diagnosis are being developed, such as fluorescence polarization assay which is a simple method for measuring antigen-antibody interaction. The aim of this study was to assess cELISA and Fluorescence Polarization Assay performance for the serological diagnosis of EIA by comparing their results with those of the Coggins test. Tests were performed on 91 workhorses from an endemic zone in the northeast region of Argentina. From the total samples analyzed, 42 tested negative and 49 tested positive in the Coggins test. Same results were obtained using FPA. Using the cELISA, 41 negative results and 50 positive results were obtained. The Receiver Operating Characteristic analysis showed that FPA performance was excellent. Therefore FPA is proposed as an outstanding EIA diagnostic test to be validated in the near future by its simplicity, speed, and objective interpretation of results.
Palabras clave:
CELISA
,
COGGINS TEST
,
FLURESCENCE POLARIZATION ASSAY
,
HORSES
Archivos asociados
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Identificadores
Colecciones
Articulos(IGEVET)
Articulos de INST.DE GENETICA VET ING FERNANDO NOEL DULOUT
Articulos de INST.DE GENETICA VET ING FERNANDO NOEL DULOUT
Citación
Espasandin, Ana Gabriela; Cipolini, María F.; Forletti, Agustina; Diaz, Silvina; Soto, Javier; et al.; Comparison of serological techniques for the diagnosis of equine infectious Anemia in an endemic area of Argentina; Elsevier Science; Journal of Virological Methods; 291; 114101; 5-2021; 1-5
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