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dc.contributor.author
Malanga, Gabriela Fabiana
dc.contributor.author
Estevez, Maria Susana
dc.contributor.author
Calvo, Juan Carlos
dc.contributor.author
Puntarulo, Susana Ángela
dc.date.available
2022-05-04T17:50:18Z
dc.date.issued
2004-09
dc.identifier.citation
Malanga, Gabriela Fabiana; Estevez, Maria Susana; Calvo, Juan Carlos; Puntarulo, Susana Ángela; Oxidative stress in limpets exposed to different environmental conditions in the Beagle Channel; Elsevier Science; Aquatic Toxicology; 69; 4; 9-2004; 299-309
dc.identifier.issn
0166-445X
dc.identifier.uri
http://hdl.handle.net/11336/156510
dc.description.abstract
The aim of this work was to study the oxidative profile of digestive glands of two limpets species (Nacella (Patinigera) magellanica and Nacella (Patinigera) deaurata) exposed to different environmental conditions. The intertidal population of N. (P.) magellanica is subjected to a wide variety of stresses not experienced by N. (P.) deaurata. Although a typical electron paramagnetic resonance (EPR) spectrum of ascorbyl radical in digestive gland from both limpets was observed, neither ascorbyl radical content nor the ascorbyl radical content/ascorbate content ratio was significantly different, suggesting that the difference in the environmental conditions did not appear to be responsible for developing alterations in the oxidative status of both organisms at the hydrophilic level (e.g. cytosol). Lipid peroxidation in the digestive glands was estimated, both as the content of thiobarbituric acid reactive substances (TBARS) and as the content of lipid radicals assessed by EPR, in both organisms. TBARS and lipid radical content were 34.8 and 36.5%, respectively, lower in N. (P.) magellanica as compared to N. (P.) deaurata. On the other hand, total iron content and the rate of generation of superoxide anion were 47.9 and 51.4%, respectively, lower in N. (P.) magellanica as compared to N. (P.) deaurata. The activity of catalase and superoxide dismutase (SOD) was 35.3 and 128.6% higher in N. (P.) magellanica as compared to N. (P.) deaurata, respectively. No significant differences were determined between the digestive glands of both molluscs regarding the content of total thiols. -Tocopherol and -carotene content were significantly lower in N. (P.) magellanica as compared to N. (P.) deaurata. A distinctive EPR signal for the adduct Fe?MGD?NO (g = 2.03 and aN = 12.5 G) was detected in the homogenates of digestive glands of both limpets. A significant difference in the content of the Fe?MGD?NO adduct in digestive glands from N. (P.) magellanica and N. (P.) deaurata (491 ± 137 and 839 ± 63 pmol/g FW, respectively) was observed. Taken as a whole, the data presented here indicated that coping with environmental stressing conditions requires a complex adjustment of the physiological metabolic pathways to ensure survival by minimizing intracellular damage. It is likely that N. (P.) magellanica has a particular evolutionary adaptation to extreme environmental conditions by keeping iron content low and antioxidant activities high.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Antioxidants
dc.subject
Beagle Channel
dc.subject
Limpets
dc.subject
Nitric oxide adducts
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Oxidative stress
dc.subject.classification
Biología Marina, Limnología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Oxidative stress in limpets exposed to different environmental conditions in the Beagle Channel
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-04-26T18:10:47Z
dc.journal.volume
69
dc.journal.number
4
dc.journal.pagination
299-309
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Malanga, Gabriela Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
dc.description.fil
Fil: Estevez, Maria Susana. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
dc.description.fil
Fil: Puntarulo, Susana Ángela. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Aquatic Toxicology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.aquatox.2004.05.008
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0166445X04001754
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