Mostrar el registro sencillo del ítem
dc.contributor.author
Luchetti, Carolina Griselda
dc.contributor.author
Lorenzo, María Soledad
dc.contributor.author
Cruzans, Paula Romina
dc.contributor.author
Ghersa, Josefina
dc.contributor.author
Maza Filippin, Melina
dc.contributor.author
Lombardo, Daniel Marcelo
dc.date.available
2022-05-02T18:53:31Z
dc.date.issued
2021
dc.identifier.citation
Effect of insulin-transferrin-selenium and metformin on the in vitrofertilization of pigs; International Symposium on Reproductive Health: overcoming barriers for research in reproduction (ISRH); Buenos Aires; Argentina; 2021; 751-751
dc.identifier.issn
0390-6663
dc.identifier.uri
http://hdl.handle.net/11336/156261
dc.description.abstract
Brief introduction: The in vitro production of pig embryos is important for both commercial purposes and biomedical studies. It has a low efficiency mainly because in vitro maturation (IVM) systems are inefficient. Supplementation of the IVM medium with insulin plus metformin(M) improves pig embryonic development. We demonstrated that insulin-transferrin-selenium (ITS) plus M increases nuclear maturation, decreases oxidative stress, increases glucose consumption and the viability of cumulus cells. Objective: Our aim was to study the IVF efficiency using ITS plus M during IVM. Methods: Porcine COC obtained by follicular aspiration from slaughterhouse ovaries were in vitro matured for 44 h in modifed M199, supplemented with hMG and dAMPc during the first 22 h. Oocytes were randomly distributed in four experimental groups containing: ITS (ITS 1 µ g/mL), M (M 10-4M), ITS + M and C (no supplement). Then matured oocytes were denuded and subjected to IVF (co-incubated 4 h with refrigerated boar semen 1 × 106 sperm/mL in modified M199). The presumptive zygotes were cultured in NCSU at 39 °C with 5% CO2, 7% O2 and 100% humidity. After 20 h from the beginning of the IVF, zygotes were fixed in paraformaldehyde 4% for 30 min, washed and stored in PBS-PVA until use. To assess fertilization parameters, zygotes were stained with Hoëchst and mounted for evaluation: penetration (penetrated/inseminated), masculine pronuclear formation (MPN/penetrated), monospermy (only one sperm head or male pronucleus/penetrated), and efficiency of fertilization (monospermic/inseminated). Data were analyzed by Fisher's exact test (significant P < 0.05). Main Outcomes and Results: The group matured with ITS + M showed a significantly higher efficiency of fertilization compared to control (ITS + M 30%, n = 43 vs C 11%, n = 45). Conclusions: The supplementation of IVM media with ITS + M is a good tool to improve oocyte quality. This is reflected in a higher IVF efficiency.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
IMR Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Porcine embryo
dc.subject
In vitro embryo production
dc.subject
Polyspermy
dc.subject
Efficiency
dc.subject.classification
Otras Ciencias Veterinarias
dc.subject.classification
Ciencias Veterinarias
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Effect of insulin-transferrin-selenium and metformin on the in vitrofertilization of pigs
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2022-04-21T16:41:52Z
dc.journal.volume
48
dc.journal.number
3
dc.journal.pagination
751-751
dc.journal.pais
Italia
dc.journal.ciudad
Roma
dc.description.fil
Fil: Luchetti, Carolina Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriologías; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; Argentina
dc.description.fil
Fil: Lorenzo, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriologías; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; Argentina
dc.description.fil
Fil: Cruzans, Paula Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; Argentina
dc.description.fil
Fil: Ghersa, Josefina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriologías; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; Argentina
dc.description.fil
Fil: Maza Filippin, Melina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; Argentina
dc.description.fil
Fil: Lombardo, Daniel Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://ceog.imrpress.com/EN/10.31083/j.ceog.2021.03.0511
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.imrpress.com/journal/CEOG/48/3/10.31083/j.ceog.2021.03.0511
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.coverage
Internacional
dc.type.subtype
Simposio
dc.description.nombreEvento
International Symposium on Reproductive Health: overcoming barriers for research in reproduction (ISRH)
dc.date.evento
2021-05-07
dc.description.ciudadEvento
Buenos Aires
dc.description.paisEvento
Argentina
dc.type.publicacion
Journal
dc.description.institucionOrganizadora
Centro de Estudios Farmacológicos y Botánicos
dc.source.revista
Clinical and experimental obstetrics and gynecology
dc.date.eventoHasta
2021-05-07
dc.type
Simposio
Archivos asociados