Show simple item record Clevenger, Josh P. Van Houten, Jason Blackwood, Michelle Rodríguez, Gustavo Rubén Jikumaru, Yusuke Kamiya, Yuji Kusano, Miyako Saito, Kazuki Visa, Sofia Van Der Knaap, Esther 2017-04-21T20:47:45Z 2015-07
dc.identifier.citation Clevenger, Josh P.; Van Houten, Jason; Blackwood, Michelle; Rodríguez, Gustavo Rubén; Jikumaru, Yusuke; et al.; Network analyses reveal shifts in transcript profiles and metabolites that accompany the expression of sun and an elongated tomato fruit; American Society Of Plant Biologist; Plant Physiology; 168; 3; 7-2015; 1164-1178
dc.identifier.issn 0032-0889
dc.description.abstract SUN controls elongated tomato (Solanum lycopersicum) shape early in fruit development through changes in cell number along the different axes of growth. The gene encodes a member of the IQ domain family characterized by a calmodulin binding motif. To gain insights into the role of SUN in regulating organ shape, we characterized genome-wide transcriptional changes and metabolite and hormone accumulation after pollination and fertilization in wild-type and SUN fruit tissues. Pericarp, seed/placenta, and columella tissues were collected at 4, 7, and 10 d post anthesis. Pairwise comparisons between SUN and the wild type identified 3,154 significant differentially expressed genes that cluster in distinct gene regulatory networks. Gene regulatory networks that were enriched for cell division, calcium/transport, lipid/hormone, cell wall, secondary metabolism, and patterning processes contributed to profound shifts in gene expression in the different fruit tissues as a consequence of high expression of SUN. Promoter motif searches identified putative cis-elements recognized by known transcription factors and motifs related to mitotic-specific activator sequences. Hormone levels did not change dramatically, but some metabolite levels were significantly altered, namely participants in glycolysis and the tricarboxylic acid cycle. Also, hormone and primary metabolite networks shifted in SUN compared with wild-type fruit. Our findings imply that SUN indirectly leads to changes in gene expression, most strongly those involved in cell division, cell wall, and patterningrelated processes. When evaluating global coregulation in SUN fruit, the main node represented genes involved in calcium-regulated processes, suggesting that SUN and its calmodulin binding domain impact fruit shape through calcium signaling.
dc.format application/pdf
dc.language.iso eng
dc.publisher American Society Of Plant Biologist
dc.rights info:eu-repo/semantics/openAccess
dc.subject FRUIT SHAPE
dc.subject SIGNALING
dc.subject.classification Biología del Desarrollo
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Network analyses reveal shifts in transcript profiles and metabolites that accompany the expression of sun and an elongated tomato fruit
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion 2017-02-23T13:55:09Z
dc.journal.volume 168
dc.journal.number 3
dc.journal.pagination 1164-1178
dc.journal.pais Estados Unidos
dc.journal.ciudad Rockville
dc.description.fil Fil: Clevenger, Josh P..
dc.description.fil Fil: Van Houten, Jason.
dc.description.fil Fil: Blackwood, Michelle.
dc.description.fil Fil: Rodríguez, Gustavo Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Jikumaru, Yusuke.
dc.description.fil Fil: Kamiya, Yuji.
dc.description.fil Fil: Kusano, Miyako.
dc.description.fil Fil: Saito, Kazuki.
dc.description.fil Fil: Visa, Sofia.
dc.description.fil Fil: Van Der Knaap, Esther.
dc.journal.title Plant Physiology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/

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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)