Measurement of ascorbic acid and glutathione content in Cyanobacterium Synechocystis sp. PCC 6803

Ascorbic acid (AsA) and gluthathione (GSH) are two key components of the antioxidant machinery of eukaryotic and prokaryotic cells. The cyanobacterium Synechocystis sp. PCC 6803 presents both compounds in different concentrations (AsA, 20-100 μM and GSH, 2-5 mM). Therefore, it is important to have precise and sensitive methods to determine the redox status in the cell and to detect variations in this antioxidants. In this protocol, we describe an improved method to estimate the content of both antioxidants (in their reduced and oxidized forms) from the same sample obtained from liquid cultures of Synechocystis sp. PCC 6803.

Palabras clave: ASCORBIC ACID , GLUTHATHIONE , CYANOBACTERIA , ANTIOXIDANTS , OXIDATIVE STRESS

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Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)

Identificadores

URI: http://hdl.handle.net/11336/156023

URL: https://bio-protocol.org/e3800

DOI: http://dx.doi.org/10.21769/BioProtoc.3800

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Articulos(INFIVE)
Articulos de INST.DE FISIOLOGIA VEGETAL

Citación

Aguilera, Anabella; Steelheart Molina, Maria Charlotte; Alegre, Matias Leonel; Berdun, Federico Juan; Salerno, Graciela Lidia; et al.; Measurement of ascorbic acid and glutathione content in Cyanobacterium Synechocystis sp. PCC 6803; Standford University; Bio-protocol; 10; 20; 20-10-2020; 1-7

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