Mostrar el registro sencillo del ítem
dc.contributor.author
Oyuela Aguilar, Monica
dc.contributor.author
Gobbi, Alex
dc.contributor.author
Browne, Patrick D.
dc.contributor.author
Ellegaard Jensen, Lea
dc.contributor.author
Hansen, Lars Hestbjerg
dc.contributor.author
Semorile, Liliana
dc.contributor.author
Pistorio, Mariano
dc.date.available
2022-04-26T19:09:38Z
dc.date.issued
2020-12-14
dc.identifier.citation
Oyuela Aguilar, Monica; Gobbi, Alex; Browne, Patrick D.; Ellegaard Jensen, Lea; Hansen, Lars Hestbjerg; et al.; Influence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of San Juan Province, Argentina; Public Library of Science; Plos One; 15; 12 ; 14-12-2020; 1-19
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/155812
dc.description.abstract
Soil microbiomes, as a primary reservoir for plant colonizing fungi and bacteria, play a major role in determining plant productivity and preventing invasion by pathogenic microorganisms. The use of 16S rRNA and ITS high-throughput amplicon sequencing for analysis of complex microbial communities have increased dramatically in recent years, establishing links between wine specificity and, environmental and viticultural factors, which are framed into the elusive terroir concept. Given the diverse and complex role these factors play on microbial soil structuring of agricultural crops, the main aim of this study is to evaluate how external factors, such as vintage, vineyard location, cultivar and soil characteristics, may affect the diversity of the microbial communities present. Additionally, we aim to compare the influence these factors have on the structuring of bacterial and fungal populations associated with Malbec grapevine rhizosphere with that of the more widespread Cabernet Sauvignon grapevine cultivar. Samples were taken from Malbec and Cabernet Sauvignon cultivars from two different vineyards in the San Juan Province of Argentina. Total DNA extracts from the rhizosphere soil samples were sequenced using Illumina’s Miseq technology, targeting the V3-V4 hypervariable 16S rRNA region in prokaryotes and the ITS1 region in yeasts. The major bacterial taxa identified were Proteobacteria, Bacteroidetes and Firmicutes, while the major fungal taxa were Ascomycetes, Basidiomycetes, Mortierellomycetes and a low percentage of Glomeromycetes. Significant differences in microbial community composition were found between vintages and vineyard locations, whose soils showed variances in pH, organic matter, and content of carbon, nitrogen, and absorbable phosphorus.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
VID
dc.subject
MICROBIOME
dc.subject
AMPLICON SEQUENCING
dc.subject
RHIZOSPHERE
dc.subject.classification
Biología Celular, Microbiología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Influence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of San Juan Province, Argentina
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-09-06T17:45:23Z
dc.journal.volume
15
dc.journal.number
12
dc.journal.pagination
1-19
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Oyuela Aguilar, Monica. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
dc.description.fil
Fil: Gobbi, Alex. Universidad de Copenhagen; Dinamarca
dc.description.fil
Fil: Browne, Patrick D.. Universidad de Copenhagen; Dinamarca
dc.description.fil
Fil: Ellegaard Jensen, Lea. University Aarhus; Dinamarca
dc.description.fil
Fil: Hansen, Lars Hestbjerg. Universidad de Copenhagen; Dinamarca
dc.description.fil
Fil: Semorile, Liliana. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
dc.description.fil
Fil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0243848
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0243848
Archivos asociados