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dc.contributor.author
Escobar, Mariana  
dc.contributor.author
Herrera Seitz, Karina  
dc.contributor.author
Studdert, Claudia Alicia  
dc.date.available
2022-04-22T16:56:21Z  
dc.date.issued
2018  
dc.identifier.citation
Bioconversion of glycerol into polyhydroxyalkanoates by a salt-tolerant indigenous strain, Halomonas titanicae KHS3; 54th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Entre Ríos; Argentina; 2018; 1-8  
dc.identifier.issn
1667-5746  
dc.identifier.uri
http://hdl.handle.net/11336/155579  
dc.description.abstract
Halomonas titanicae KHS3 was isolated from hydrocarbon-contaminated water in Mar del Plata harbor. This strain is able to accumulate polyhydroxyalkanoates (PHAs), reserve polymers that can be used as raw material for the preparation of bioplastics.The aim of this work was to evaluate the ability of H. titanicae KHS3 to use glycerol as the only source of carbon and energy and convert it into PHAs.When grown in mineral salts medium with 0.25% commercial glycerol, PHAs synthesis was only moderate. However, when cells were harvested at mid-exponential phase and resuspended in medium depleted of nitrogen source, PHA accumulation was dramatically increased and reached up to 70% of dry cell weight. Such accumulation also occurred when cultures were fed with glycerol obtained from a biodiesel reactor, both in its crude form (contaminated with methanol and salts) and partially purified (technical grade), suggesting that this bioconversion potentially represents a way of adding value to the otherwise disposable glycerol.We show the kinetics of PHA accumulation after nitrogen deprivation under different conditions. Good PHA accumulation occurred in media containing between 2 and 10% NaCl. PHAs inside cells remained stable after long incubations in nitrogen-lacking medium, but decreased rapidly after re-addition of ammonium sulfate.The purified polymer is currently analyzed by RMN to determine its precise chemical composition.  
dc.format
application/pdf  
dc.language.iso
spa  
dc.publisher
Biocell  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Halomonas  
dc.subject
PHAs  
dc.subject
glicerol  
dc.subject.classification
Biología Celular, Microbiología  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Bioconversion of glycerol into polyhydroxyalkanoates by a salt-tolerant indigenous strain, Halomonas titanicae KHS3  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/conferenceObject  
dc.type
info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2022-03-17T14:08:25Z  
dc.journal.volume
42  
dc.journal.number
Suppl 4  
dc.journal.pagination
1-8  
dc.journal.pais
Argentina  
dc.description.fil
Fil: Escobar, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina  
dc.description.fil
Fil: Herrera Seitz, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina  
dc.description.fil
Fil: Studdert, Claudia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2018.pdf  
dc.conicet.rol
Autor  
dc.conicet.rol
Autor  
dc.conicet.rol
Autor  
dc.coverage
Nacional  
dc.type.subtype
Congreso  
dc.description.nombreEvento
54th Annual Meeting Argentine Society for Biochemistry and Molecular Biology  
dc.date.evento
2018-11-05  
dc.description.ciudadEvento
Entre Ríos  
dc.description.paisEvento
Argentina  
dc.type.publicacion
Journal  
dc.description.institucionOrganizadora
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular  
dc.source.revista
54th Annual Meeting Argentine Society for Biochemistry and Molecular Biology 54 Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecualr  
dc.date.eventoHasta
2018-11-08  
dc.type
Congreso