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dc.contributor.author
Villarreal, Natalia Marina  
dc.contributor.author
Rosli, Hernan Guillermo  
dc.contributor.author
Martinez, Gustavo Adolfo  
dc.contributor.author
Civello, Pedro Marcos  
dc.date.available
2022-04-12T19:50:18Z  
dc.date.issued
2008-02  
dc.identifier.citation
Villarreal, Natalia Marina; Rosli, Hernan Guillermo; Martinez, Gustavo Adolfo; Civello, Pedro Marcos; Polygalacturonase activity and expression of related genes during ripening of strawberry cultivars with contrasting fruit firmness; Elsevier Science; Postharvest Biology and Technology; 47; 2; 2-2008; 141-150  
dc.identifier.issn
0925-5214  
dc.identifier.uri
http://hdl.handle.net/11336/155127  
dc.description.abstract
Fleshy fruits soften during ripening mainly as a consequence of the catabolism of cell wall components. In strawberry (Fragaria × ananassa Duch), the depolymerization and solubilization of pectins increase during ripening and contribute to fruit softening. In the present paper, we report the cloning and expression analysis of two polygalacturonase (PG) putative cDNAs: FaPG1 and T-PG. The former seems to be the same sequence of previously reported PG in strawberry, while T-PG cDNA has a deletion of 85 bp that cause a frame shift and would produce an inactive protein. Measurement of total PG activity and expression of FaPG1 and T-PG were performed in strawberry cultivars with contrasting softening rates. The softest cultivar (Toyonaka) showed the higher total PG activity in all ripening stages analyzed. The analysis by RT-PCR revealed that both genes express in the three cultivars, though the expression pattern was different. In the firmer cultivars (Selva and Camarosa) the expression of T-PG was considerably higher than the expression of FaPG1, while the opposite occurred in the softest cultivar (Toyonaka). Therefore, the higher PG activity detected in Toyonaka correlates with the enhanced expression of FaPG1 gene, while the low PG activity found in the firm cultivars correlates with a higher expression of T-PG, a gene that could encode a truncated protein without PG activity. The influence of auxins on both the expression of PG genes and the total PG activity during strawberry fruit ripening was also analyzed.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
CELL WALL  
dc.subject
FRAGARIA  
dc.subject
PECTIN  
dc.subject
POLYGALACTURONASE  
dc.subject
SOFTENING  
dc.subject.classification
Alimentos y Bebidas  
dc.subject.classification
Otras Ingenierías y Tecnologías  
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS  
dc.title
Polygalacturonase activity and expression of related genes during ripening of strawberry cultivars with contrasting fruit firmness  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2021-12-03T19:34:44Z  
dc.journal.volume
47  
dc.journal.number
2  
dc.journal.pagination
141-150  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Villarreal, Natalia Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina  
dc.description.fil
Fil: Rosli, Hernan Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina  
dc.description.fil
Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina  
dc.description.fil
Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina  
dc.journal.title
Postharvest Biology and Technology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.postharvbio.2007.06.011  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0925521407002177?via%3Dihub