Evento
A proposed reaction mechanism for maize monomeric glyoxalase I
Gonzalez, Javier Marcelo
; Agostini, Romina Belén
; Alvarez, Clarisa Ester
; Klinke, Sebastian
; Andreo, Carlos Santiago
; Campos Bermudez, Valeria Alina
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Tipo del evento:
Reunión
Nombre del evento:
XLVIII Reunión Anual de la Sociedad Argentina de Biofísica
Fecha del evento:
27/11/2019
Institución Organizadora:
Sociedad Argentina de Biofísica;
Universidad Nacional de San Luis;
Título del Libro:
XLVIII Reunión Anual de la Sociedad Argentina de Biofísica: Libro de resúmenes
Editorial:
Sociedad Argentina de Biofísica
ISBN:
978-987-27591-7-9
Idioma:
Inglés
Clasificación temática:
Resumen
Detoxification of methylglyoxal, a toxic by-product of central sugar metabolism, is a major issue for all forms of life. The glyoxalase pathway evolved to effectively convert methylglyoxal into D-lactate via a glutathione hemithioacetal intermediate. Recently, we have shown that the monomeric glyoxalase I from maize exhibits a symmetric fold with two cavities, potentially harboring two active sites, in analogy with homodimeric enzyme surrogates. Here we confirm that only one of the two cavities exhibits glyoxalase I activity and show that it adopts a tunnel-shaped structure upon substrate binding. Such conformational change gives rise to independent binding sites for glutathione and methylglyoxal in the same active site, with important implications for the molecular reaction mechanism, which has been a matter of debate for several decades.
Palabras clave:
METHYLGLYOXAL
,
GLYOXALASE I
,
X-RAY STRUCTURE
Archivos asociados
Licencia
Identificadores
Colecciones
Eventos(CEFOBI)
Eventos de CENTRO DE EST.FOTOSINTETICOS Y BIOQUIMICOS (I)
Eventos de CENTRO DE EST.FOTOSINTETICOS Y BIOQUIMICOS (I)
Eventos(IIBBA)
Eventos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Eventos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Eventos(INBIONATEC)
Eventos de INSTITUTO DE BIONANOTECNOLOGIA DEL NOA
Eventos de INSTITUTO DE BIONANOTECNOLOGIA DEL NOA
Citación
A proposed reaction mechanism for maize monomeric glyoxalase I; XLVIII Reunión Anual de la Sociedad Argentina de Biofísica; San Luis; Argentina; 2019; 71-71
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