Evento
Mucins and polysaccharides from Echinococcus granulosus laminar layer induce a mild maturation phenotype in dendritic cells and promote gene expression of pro-inflammatory cytokines
Plá, Natalia; Chop, Maia
; Nicolao, María Celeste
; Cumino, Andrea Carina
; Rodríguez Rodrígues, Christian Fernando Ariel
Tipo del evento:
Reunión
Nombre del evento:
LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología
Fecha del evento:
10/11/2020
Institución Organizadora:
Sociedad Argentina de Investigación Clínica;
Sociedad Argentina de Inmunología;
Sociedad Argentina de Fisiología;
Título de la revista:
Medicina (Buenos Aires)
Editorial:
Fundación Revista Medicina
ISSN:
0025-7680
e-ISSN:
1669-9106
Idioma:
Inglés
Clasificación temática:
Resumen
Background: The laminar layer is an acellular structure rich in glycans that surrounds the metacestode, creating a mechanical and immunological protective barrier, crucial in the Echinococcus?host interface. The aim of this work is to analyze if purified laminar layer (pLL) could trigger maturation and cytokine production in dendritic cells by activating mTOR pathway, a central regulator of cell metabolism and environmental signals. Methods: BMDCs were cultured in complete RPMI supplemented with 100 ng/ml Flt3-L.The germinal layer was removed from pLL by washing with 2M NaCl. Flow cytometry: FITC or PE-conjugated mAbs directed to CD11c, CD40, CD80, CD86, MHC I and MHC II were used. Immunobloting and confocal microscopy: mouse anti-puromycin and rabbit anti-Phospho-mTOR antibodies were used. Gene expression analysis of IL-10, IL-12p35, IL-6, TNF-, IL-23p19, TGF- was performed on a Real Time PCR System Results: The pLL of Echinococcus induce a non-statistically significant, but a trend in the up-regulation of CD86 and MHC II and that expression change was diminished by the use of rapamycin (n=5). Conversely, no changes in the expression of CD40, CD80 or MHC I were registered. Further studies were done to analyze whether pLL was also able to stimulate the production of cytokines by BMDCs. When BMDCs were stimulated with pLL induce the expression of IL-6 and TNF-an=3 *p<0.05, **p<0.01), but not differences compared to control were observed in IL-12, IL-23, IL-10 and TGF-. Finally, we evaluated global protein synthesis and phosphorylation of mTOR using confocal microscopy or western blot. pLL (MFI:_979) showed an increase in translation levels compared to its counterpart without stimulation (MFI:_686, *p<0.05 n=3) and also induce an increase in mTOR phosphorylation levels compared to untreated BMCDs (n=3, p<0.01) Conclusions: These data suggest that Echinococcus pLL are recognized by DCs and induce activation of mTOR pathway favoring cell activation.
Palabras clave:
Mucins
,
laminar layer
,
Echinococcus granulosus
,
dendritic cells
Archivos asociados
Licencia
Identificadores
Colecciones
Eventos (IIPROSAM)
Eventos de INSTITUTO DE INVESTIGACIONES EN PRODUCCION, SANIDAD Y AMBIENTE
Eventos de INSTITUTO DE INVESTIGACIONES EN PRODUCCION, SANIDAD Y AMBIENTE
Citación
Mucins and polysaccharides from Echinococcus granulosus laminar layer induce a mild maturation phenotype in dendritic cells and promote gene expression of pro-inflammatory cytokines; LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2020; 153-154
Compartir