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dc.contributor.author
Albacete, Alfonso
dc.contributor.author
Cantero Navarro, Elena
dc.contributor.author
Großkinsky, Dominik K.
dc.contributor.author
Arias, Cintia Lucia
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dc.contributor.author
Balibrea, María Encarnación
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Bru, Roque
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Fragner, Lena
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Ghanem, Michel E.
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González, María de la Cruz
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Hernández, José A.
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Martínez Andújar, Cristina
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van der Graaff, Eric
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Weckwerth, Wolfram
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Zellnig, Günther
dc.contributor.author
Francisco Pérez Alfocea
dc.contributor.author
Roitsch, Thomas
dc.date.available
2017-04-19T15:30:54Z
dc.date.issued
2014-11
dc.identifier.citation
Albacete, Alfonso; Cantero Navarro, Elena; Großkinsky, Dominik K.; Arias, Cintia Lucia; Balibrea, María Encarnación; et al.; Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato; Oxford University Press; Journal Of Experimental Botany; 66; 3; 11-2014; 863-878
dc.identifier.issn
0022-0957
dc.identifier.uri
http://hdl.handle.net/11336/15445
dc.description.abstract
Drought stress conditions modify source–sink relations, thereby influencing plant growth, adaptive responses, and consequently crop yield. Invertases are key metabolic enzymes regulating sink activity through the hydrolytic cleavage of sucrose into hexose monomers, thus playing a crucial role in plant growth and development. However, the physiological role of invertases during adaptation to abiotic stress conditions is not yet fully understood. Here it is shown that plant adaptation to drought stress can be markedly improved in tomato (Solanum lycopersicum L.) by overexpression of the cell wall invertase (cwInv) gene CIN1 from Chenopodium rubrum. CIN1 overexpression limited stomatal conductance under normal watering regimes, leading to reduced water consumption during the drought period, while photosynthetic activity was maintained. This caused a strong increase in water use efficiency (up to 50%), markedly improving water stress adaptation through an efficient physiological strategy of dehydration avoidance. Drought stress strongly reduced cwInv activity and induced its proteinaceous inhibitor in the leaves of the wild-type plants. However, the CIN1-overexpressing plants registered 3- to 6-fold higher cwInv activity in all analysed conditions. Surprisingly, the enhanced invertase activity did not result in increased hexose concentrations due to the activation of the metabolic carbohydrate fluxes, as reflected by the maintenance of the activity of key enzymes of primary metabolism and increased levels of sugar-phosphate intermediates under water deprivation. The induced sink metabolism in the leaves explained the maintenance of photosynthetic activity, delayed senescence, and increased source activity under drought stress. Moreover, CIN1 plants also presented a better control of production of reactive oxygen species and sustained membrane protection. Those metabolic changes conferred by CIN1 overexpression were accompanied by increases in the concentrations of the senescence-delaying hormone trans-zeatin and decreases in the senescence-inducing ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) in the leaves. Thus, cwInv critically functions at the integration point of metabolic, hormonal, and stress signals, providing a novel strategy to overcome drought-induced limitations to crop yield, without negatively affecting plant fitness under optimal growth conditions.
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application/pdf
dc.language.iso
eng
dc.publisher
Oxford University Press
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cell Wall Invertase
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Cytokinins
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Drought Stress
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Tomato
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Ethylene
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Sourcesink Relationships
dc.subject.classification
Ciencias de las Plantas, Botánica
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-04-18T14:02:33Z
dc.journal.volume
66
dc.journal.number
3
dc.journal.pagination
863-878
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Oxford
dc.description.fil
Fil: Albacete, Alfonso. Consejo Superior de Investigaciones Cientificas. Centro de Edafologia y Biologia Aplic.del Segura; España. University of Graz; Austria
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Fil: Cantero Navarro, Elena. Consejo Superior de Investigaciones Cientificas. Centro de Edafologia y Biologia Aplic.del Segura; España
dc.description.fil
Fil: Großkinsky, Dominik K.. University of Graz; Austria. Universidad de Copenhagen; Dinamarca
dc.description.fil
Fil: Arias, Cintia Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; Argentina
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Fil: Balibrea, María Encarnación. Consejo Superior de Investigaciones Cientificas. Centro de Edafologia y Biologia Aplic.del Segura; España
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Fil: Bru, Roque. Universidad de Alicante; España
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Fil: Fragner, Lena. Universidad de Viena; Austria
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Fil: Ghanem, Michel E.. Consejo Superior de Investigaciones Cientificas. Centro de Edafologia y Biologia Aplic.del Segura; España
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Fil: González, María de la Cruz. Universidad de Sevilla; España
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Fil: Hernández, José A.. Consejo Superior de Investigaciones Cientificas. Centro de Edafologia y Biologia Aplic.del Segura; España
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Fil: Martínez Andújar, Cristina. Consejo Superior de Investigaciones Cientificas. Centro de Edafologia y Biologia Aplic.del Segura; España
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Fil: van der Graaff, Eric. University of Graz; Austria. Universidad de Copenhagen; Dinamarca
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Fil: Weckwerth, Wolfram. Universidad de Viena; Austria
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Fil: Zellnig, Günther. University of Graz; Austria
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Fil: Francisco Pérez Alfocea. Consejo Superior de Investigaciones Cientificas. Centro de Edafologia y Biologia Aplic.del Segura; España
dc.description.fil
Fil: Roitsch, Thomas. University of Graz; Austria. Universidad de Copenhagen; Dinamarca. Global Change Research Centre; República Checa
dc.journal.title
Journal Of Experimental Botany
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/jxb/eru448
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/eru448
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4321548/
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