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dc.contributor.author
Casati, Paula
dc.contributor.author
Campi, Mabel
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Morrow, Darren J.
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Fernandes, John F.
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Walbot, Virginia
dc.date.available
2017-04-18T21:25:38Z
dc.date.issued
2011-06
dc.identifier.citation
Casati, Paula; Campi, Mabel; Morrow, Darren J.; Fernandes, John F.; Walbot, Virginia; Transcriptomic, proteomic and metabolomic analysis of UV-B signaling in maize; Biomed Central; Bmc Genomics; 12; 321; 6-2011; 1-17
dc.identifier.issn
1471-2164
dc.identifier.uri
http://hdl.handle.net/11336/15424
dc.description.abstract
Background: Under normal solar fluence, UV-B damages macromolecules, but it also elicits physiological acclimation and developmental changes in plants. Excess UV-B decreases crop yield. Using a treatment twice solar fluence, we focus on discovering signals produced in UV-B-irradiated maize leaves that translate to systemic changes in shielded leaves and immature ears. Results: Using transcriptome and proteomic profiling, we tracked the kinetics of transcript and protein alterations in exposed and shielded organs over 6 h. In parallel, metabolic profiling identified candidate signaling molecules based on rapid increase in irradiated leaves and increased levels in shielded organs; pathways associated with the synthesis, sequestration, or degradation of some of these potential signal molecules were UV-B-responsive. Exposure of just the top leaf substantially alters the transcriptomes of both irradiated and shielded organs, with greater changes as additional leaves are irradiated. Some phenylpropanoid pathway genes are expressed only in irradiated leaves, reflected in accumulation of pathway sunscreen molecules. Most protein changes detected occur quickly: approximately 92% of the proteins in leaves and 73% in immature ears changed after 4 h UV-B were altered by a 1 h UV-B treatment. Conclusions: There were significant transcriptome, proteomic, and metabolomic changes under all conditions studied in both shielded and irradiated organs. A dramatic decrease in transcript diversity in irradiated and shielded leaves occurs between 0 h and 1 h, demonstrating the susceptibility of plants to short term UV-B spikes as during ozone depletion. Immature maize ears are highly responsive to canopy leaf exposure to UV-B.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Biomed Central
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Maize
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Uv-B
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Transcriptome
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Proteome
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Metabolome
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Transcriptomic, proteomic and metabolomic analysis of UV-B signaling in maize
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-04-18T14:01:58Z
dc.journal.volume
12
dc.journal.number
321
dc.journal.pagination
1-17
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
dc.description.fil
Fil: Campi, Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
dc.description.fil
Fil: Morrow, Darren J.. University Of Stanford; Estados Unidos
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Fil: Fernandes, John F.. University Of Stanford; Estados Unidos
dc.description.fil
Fil: Walbot, Virginia. University Of Stanford; Estados Unidos
dc.journal.title
Bmc Genomics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/1471-2164-12-321
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-12-321
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141669/


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