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Evento

Evidence of tryglyceride-phase incorporation into artificial bilayers for studying lipid droplets biogenesis

Caruso, BenjaminIcon ; Mangiarotti, AgustínIcon ; Wilke, NataliaIcon ; Perillo, Maria AngelicaIcon
Colaboradores: Celej, Maria SoledadIcon
Tipo del evento: Reunión
Nombre del evento: XLVII Reunión Anual de la Sociedad Argentina de Biofísica
Fecha del evento: 05/12/2018
Institución Organizadora: Sociedad Argentina de Biofísica; Universidad Nacional de La plata. Facultad de Ciencias Médicas;
Título del Libro: XLVII Reunión Anual de la Sociedad Argentina de Biofísica: Libro de resúmenes
Editorial: Sociedad Argentina de Biofísica
ISBN: 978-987-27591-6-2
Idioma: Inglés
Clasificación temática:
Biofísica

Resumen

Lipid Droplets (LD) are intracelular structures consisting on an apolar lipid core - composed mainly of triglycerides (TG) and steryl esters- which is surrounded by a phospholipid and protein monolayer. LDs originate in the ER bilayer, where TG synthesis concludes. The mechanisms underlying TG nucleation, size maduration and budding-off from the ER membrane are a matter of current investigations and the role of dewetting from cytosolic-bilayer interface appears to play a critical role. In order to contrast the nano-sized "blisters" of TG that some authors predict1, here we formed free-standing bilayers by transferring films of a monolayer of mixed phosphatidylcholine(EPC)/TG in coexistence with TG microlenses (i.e. an excluded TG phase floating in the surface). These membranes were characterized by adding them the solvatochromic fluorescent probe Nile Red (NR) and observing them under spectral confocal microscope. Such bilayers exhibit fluorescence emission spectra comparable of bilayers of vesicles with similar composition (POPC and TO). By comparison with literature data and fluorescence spectra of EPC and TG monolayers, the peaks could be assigned to different phases, namely 1) PC membranes (λemmax=630 nm ) bilayer and bilayer) and 2) TG isotropic phase (λemmax=570 nm ). No microscopic structures could be observed at λ emmax=570 nm. Diffusion of NR under this TG phase was characterized using FRAP analysis yielding values (D=2 μm2s ) typical of model bilayer membranes, suggesting that the probe is diffusing in a 2D structure. This system appears appropiate for describing which is the distribution of the TG phase, that is, homogeneously among the intrabilayer space or in nanoscopic "blisters", by evaluating diffusion times obtained by FCS and FRAP.
Palabras clave: Biomembranas , Lipid Droplets , Fluorescence Correlation Spectroscopy
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/154225
URL: https://biofisica.org.ar/reuniones-cientificas/reunionsab-previas/
URL: https://biofisica.org.ar/xlii-reunion-anual-sab/
Colecciones
Eventos(CIQUIBIC)
Eventos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Eventos(IIBYT)
Eventos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS Y TECNOLOGICAS
Citación
Evidence of tryglyceride-phase incorporation into artificial bilayers for studying lipid droplets biogenesis; XLVII Reunión Anual de la Sociedad Argentina de Biofísica; La Plata; Argentina; 2018; 75-75
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