Mostrar el registro sencillo del ítem

dc.contributor.author
Islam, Md Aminul  
dc.contributor.author
Albarracín, Leonardo Miguel  
dc.contributor.author
Melnikov, Vyacheslav  
dc.contributor.author
Andrade, Bruno G. N.  
dc.contributor.author
Cuadrat, Rafael R. C.  
dc.contributor.author
Kitazawa, Haruki  
dc.contributor.author
Villena, Julio Cesar  
dc.date.available
2022-03-23T12:00:51Z  
dc.date.issued
2021-05  
dc.identifier.citation
Islam, Md Aminul; Albarracín, Leonardo Miguel; Melnikov, Vyacheslav; Andrade, Bruno G. N.; Cuadrat, Rafael R. C.; et al.; Dolosigranulum pigrum modulates immunity against SARS-CoV-2 in respiratory epithelial cells; MDPI AG; Pathogens; 10; 6; 5-2021; 1-11  
dc.identifier.issn
2076-0817  
dc.identifier.uri
http://hdl.handle.net/11336/153800  
dc.description.abstract
In a previous work, we demonstrated that nasally administered Dolosigranulum pigrum 040417 beneficially modulated the respiratory innate immune response triggered by the activation of Toll-like receptor 3 (TLR3) and improved protection against Respiratory Syncytial Virus (RSV) in mice. In this work, we aimed to evaluate the immunomodulatory effects of D. pigrum 040417 in human respiratory epithelial cells and the potential ability of this immunobiotic bacterium to in-crease the protection against Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The respiratory commensal bacterium D. pigrum 040417 differentially modulated the production of IFN-β, IL-6, CXCL8, CCL5 and CXCL10 in the culture supernatants of Calu-3 cells stimulated with poly(I:C) or challenged with SARS-CoV-2. The differential cytokine profile induced by the 040417 strain was associated with a significant reduction in viral replication and cellular damage after coronavirus infection. Of note, D. pigrum 030918 was not able to modify the resistance of Calu-3 cells to SARS-CoV-2 infection, indicating a strain-specific immunomodulatory effect for respiratory commensal bacteria. The findings of this work improve our understanding of the immunological mechanisms involved in the modulation of respiratory immunity induced by respiratory commensal bacteria, by demonstrating their specific effect on respiratory epithelial cells. In addition, the results suggest that particular strains such as D. pigrum 040417 could be used as a promising alternative for combating SARS-CoV-2 and reducing the severity of COVID-19.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
MDPI AG  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/  
dc.subject
CORONAVIRUS  
dc.subject
COVID-19  
dc.subject
DOLOSIGRANULUM PIGRUM  
dc.subject
NEXT GENERATION PROBIOTICS  
dc.subject
RESPIRATORY COMMENSAL BACTERIA  
dc.subject
RESPIRATORY EPITHELIAL CELLS  
dc.subject
SARS-COV-2  
dc.subject.classification
Inmunología  
dc.subject.classification
Medicina Básica  
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Dolosigranulum pigrum modulates immunity against SARS-CoV-2 in respiratory epithelial cells  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2021-12-03T20:13:19Z  
dc.identifier.eissn
2076-0817  
dc.journal.volume
10  
dc.journal.number
6  
dc.journal.pagination
1-11  
dc.journal.pais
Suiza  
dc.journal.ciudad
Basel  
dc.description.fil
Fil: Islam, Md Aminul. Tohoku University; Japón. Bangladesh Agricultural University; Bangladesh  
dc.description.fil
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina  
dc.description.fil
Fil: Melnikov, Vyacheslav. Gabrichevsky Research Institute for Epidemiology and Microbiology; Rusia  
dc.description.fil
Fil: Andrade, Bruno G. N.. Munster Technological University; Irlanda  
dc.description.fil
Fil: Cuadrat, Rafael R. C.. Berlín Institute for Medical Systems Biology; Alemania  
dc.description.fil
Fil: Kitazawa, Haruki. Tohoku University; Japón  
dc.description.fil
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina  
dc.journal.title
Pathogens  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/pathogens10060634  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-0817/10/6/634