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dc.contributor.author
Islam, Md Aminul
dc.contributor.author
Albarracín, Leonardo Miguel
dc.contributor.author
Melnikov, Vyacheslav
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Andrade, Bruno G. N.
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Cuadrat, Rafael R. C.
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Kitazawa, Haruki
dc.contributor.author
Villena, Julio Cesar
dc.date.available
2022-03-23T12:00:51Z
dc.date.issued
2021-05
dc.identifier.citation
Islam, Md Aminul; Albarracín, Leonardo Miguel; Melnikov, Vyacheslav; Andrade, Bruno G. N.; Cuadrat, Rafael R. C.; et al.; Dolosigranulum pigrum modulates immunity against SARS-CoV-2 in respiratory epithelial cells; MDPI AG; Pathogens; 10; 6; 5-2021; 1-11
dc.identifier.issn
2076-0817
dc.identifier.uri
http://hdl.handle.net/11336/153800
dc.description.abstract
In a previous work, we demonstrated that nasally administered Dolosigranulum pigrum 040417 beneficially modulated the respiratory innate immune response triggered by the activation of Toll-like receptor 3 (TLR3) and improved protection against Respiratory Syncytial Virus (RSV) in mice. In this work, we aimed to evaluate the immunomodulatory effects of D. pigrum 040417 in human respiratory epithelial cells and the potential ability of this immunobiotic bacterium to in-crease the protection against Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The respiratory commensal bacterium D. pigrum 040417 differentially modulated the production of IFN-β, IL-6, CXCL8, CCL5 and CXCL10 in the culture supernatants of Calu-3 cells stimulated with poly(I:C) or challenged with SARS-CoV-2. The differential cytokine profile induced by the 040417 strain was associated with a significant reduction in viral replication and cellular damage after coronavirus infection. Of note, D. pigrum 030918 was not able to modify the resistance of Calu-3 cells to SARS-CoV-2 infection, indicating a strain-specific immunomodulatory effect for respiratory commensal bacteria. The findings of this work improve our understanding of the immunological mechanisms involved in the modulation of respiratory immunity induced by respiratory commensal bacteria, by demonstrating their specific effect on respiratory epithelial cells. In addition, the results suggest that particular strains such as D. pigrum 040417 could be used as a promising alternative for combating SARS-CoV-2 and reducing the severity of COVID-19.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
MDPI AG
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
CORONAVIRUS
dc.subject
COVID-19
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DOLOSIGRANULUM PIGRUM
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NEXT GENERATION PROBIOTICS
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RESPIRATORY COMMENSAL BACTERIA
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RESPIRATORY EPITHELIAL CELLS
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SARS-COV-2
dc.subject.classification
Inmunología
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Dolosigranulum pigrum modulates immunity against SARS-CoV-2 in respiratory epithelial cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-12-03T20:13:19Z
dc.identifier.eissn
2076-0817
dc.journal.volume
10
dc.journal.number
6
dc.journal.pagination
1-11
dc.journal.pais
Suiza
dc.journal.ciudad
Basel
dc.description.fil
Fil: Islam, Md Aminul. Tohoku University; Japón. Bangladesh Agricultural University; Bangladesh
dc.description.fil
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Melnikov, Vyacheslav. Gabrichevsky Research Institute for Epidemiology and Microbiology; Rusia
dc.description.fil
Fil: Andrade, Bruno G. N.. Munster Technological University; Irlanda
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Fil: Cuadrat, Rafael R. C.. Berlín Institute for Medical Systems Biology; Alemania
dc.description.fil
Fil: Kitazawa, Haruki. Tohoku University; Japón
dc.description.fil
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.journal.title
Pathogens
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/pathogens10060634
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-0817/10/6/634
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