Artículo
UHPLC-HESI-OT-MS-MS biomolecules profiling, antioxidant and antibacterial activity of the “orange-yellow resin” from zuccagnia punctata Cav
Gómez Pelaytay, Jessica Belén
; Simirgiotis, Mario Juan
; Manrique, Sofia
; Lima, Beatriz Viviana
; Bórquez, Jorge; Feresin, Gabriela Egly
; Tapia, Aníbal Alejandro
Fecha de publicación:
01/02/2020
Editorial:
Multidisciplinary Digital Publishing Institute
Revista:
Antioxidants
ISSN:
2076-3921
e-ISSN:
2076-3921
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
This research was designed to investigate the metabolite profiling, phenolics, and flavonoids content as well as the potential antioxidant and antibacterial, properties of orange-yellow resin from Zuccagnia punctata Cav (ZpRe). Metabolite profiling was obtained by a ultrahigh resolution liquid chromatography orbitrap MS analysis (UHPLC-ESI-OT-MS-MS). The antioxidant properties were screened by four methods: 2,2-diphenyl-1-picrylhydrazyl assay (DPPH), trolox equivalent antioxidant activity assay (TEAC), ferric-reducing antioxidant power assay (FRAP), and lipid peroxidation in erythrocytes (LP)). The antibacterial activity was evaluated according to the Clinical and Laboratory Standards Institute (CLSI) rules. The resin displayed a strong DPPH scavenging activity (IC50 = 25.72 µg/mL) and showed a percentage of inhibition of LP close to that of the reference compound catechin (70% at 100 µg ZpRe/mL), while a moderated effect was observed in the FRAP and TEAC assays. The resin showed a content of phenolic and flavonoid compounds of 391 mg GAE/g and 313 mg EQ/g respectively. Fifty phenolics compounds were identified by ultrahigh resolution liquid chromatography orbitrap MS analysis (UHPLC-PDA-OT-MS) analysis. Thirty-one compounds are reported for the first time, updating the knowledge on the chemical profile of this species. The importance of the biomolecules identified support traditional use of this endemic plant. Furthermore, additional pharmacological data is presented that increase the potential interest of this plant for industrial sustainable applications.
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Identificadores
Colecciones
Articulos(CCT - SAN JUAN)
Articulos de CENTRO CIENTIFICO TECNOLOGICO CONICET - SAN JUAN
Articulos de CENTRO CIENTIFICO TECNOLOGICO CONICET - SAN JUAN
Citación
Gómez Pelaytay, Jessica Belén; Simirgiotis, Mario Juan; Manrique, Sofia; Lima, Beatriz Viviana; Bórquez, Jorge; et al.; UHPLC-HESI-OT-MS-MS biomolecules profiling, antioxidant and antibacterial activity of the “orange-yellow resin” from zuccagnia punctata Cav; Multidisciplinary Digital Publishing Institute; Antioxidants; 9; 2; 1-2-2020; 1-17
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