Artículo
Trypanosoma cruzi infection in Cyclophilin D deficient mice
Milduberger, Natalia Ayelen; Bustos, Patricia Laura
; González, Carolina
; Perrone, Alina Elizabeth
; Postan, Miriam
; Bua, Jacqueline Elena
Fecha de publicación:
01/2021
Editorial:
Academic Press Inc Elsevier Science
Revista:
Experimental Parasitology
ISSN:
0014-4894
e-ISSN:
1090-2449
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Trypanosoma cruzi is the causative agent of Chagas disease, which is endemic in Latin America and around the world through mother to child transmission. The heart is the organ most frequently affected in the chronic stage of the human infection and depends on mitochondria for the required energy for its activity. Cyclophilins are involved in protein folding and the mitochondrial isoform, Cyclophilin D (CyPD), has a crucial role in the opening of the mitochondrial permeability transition pore. In the present study, we infected CyPD deficient mice, with ablation of the Ppif gene, with T. cruzi parasites and the course of the infection was analyzed. Parasite load, quantified by PCR, was significantly lower in skeletal and cardiac tissues of Ppif -/- mice compared to wild type mice. In vitro cultured cardiomyocytes and macrophages from mice lacking CyPD exhibited lower percentage of infected cells and number of intracellular parasites than those observed for wild type mice. Although histopathological analysis of heart and mRNA of heart cytokines showed differences between T. cruzi-infected mice compared to the uninfected animals, no significant differences were found mice due to the ablation of the Ppif gene. Our results suggest that cells deficient for mitochondrial CyPD, inhibited for the mitochondrial membrane potential collapse, reduces the severity of parasite aggression and spread of cellular infection.
Palabras clave:
CHAGAS DISEASE
,
CYCLOPHILIN D
,
MITOCHONDRIA
,
PPIF KO MICE
,
TRYPANOSOMA CRUZI
Archivos asociados
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Identificadores
Colecciones
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Milduberger, Natalia Ayelen; Bustos, Patricia Laura; González, Carolina; Perrone, Alina Elizabeth; Postan, Miriam; et al.; Trypanosoma cruzi infection in Cyclophilin D deficient mice; Academic Press Inc Elsevier Science; Experimental Parasitology; 220; 108044; 1-2021; 1-7
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