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dc.contributor.author
Gholivand, Mohammad Bagher
dc.contributor.author
Jalalvand, Ali R.
dc.contributor.author
Goicoechea, Hector Casimiro
dc.date.available
2017-04-18T16:08:10Z
dc.date.issued
2014-06
dc.identifier.citation
Gholivand, Mohammad Bagher; Jalalvand, Ali R.; Goicoechea, Hector Casimiro; Multivariate analysis for resolving interactions of carbidopa with dsDNA at a fullerene-C60/GCE; Elsevier Science; International Journal Of Biological Macromolecules; 69; 6-2014; 369-381
dc.identifier.issn
0141-8130
dc.identifier.uri
http://hdl.handle.net/11336/15364
dc.description.abstract
For the first time, interactions of carbidopa (CD) with double-stranded calf thymus DNA (dsDNA) in a phosphate buffered solution (PBS, 0.05 M, pH = 4.0) at a fullerene-C60/glassy carbon electrode (FLR/GCE) has been studied by cyclic voltammetry (CV), linear sweep voltammetry (LSV), and square wave voltammetry (SWV). The interaction of CD with dsDNA was also monitored using fluorescence (F) and UVvis spectroscopic techniques. New information was obtained when a row- and column-wise augmented matrix consisting of SWV, LSV, F and UVvis data was resolved using multivariate curve resolution-alternating least squares (MCR-ALS) as a powerful chemometric tool. Pure electrochemical and spectroscopic signals of CD, dsDNA and dsDNA-CD2 complex, and their concentration profiles were then successfully resolved. Molecular docking studies confirmed that the binding of CD with dsDNA shows minor groove binding mode which was in accordance with experimental results. Under optimized conditions, the SWV responses were linearly related to dsDNA concentration between 0.1 and 25.0 nM and a limit of detection (LOD) of 0.03 nM was calculated (3Sb/b = 3). Moreover, the modified electrode exhibited long term stability, good repeatability, and reproducibility, and high sensitivity and selectivity towards dsDNA determination in human serum samples, demonstrating its feasibility towards dsDNA sensing.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Carbidopa
dc.subject
Dsdna
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Voltametry
dc.subject
Spectroscopy
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Fullerene C60
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Chemometrics
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Molecular Docking
dc.subject.classification
Química Analítica
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Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Multivariate analysis for resolving interactions of carbidopa with dsDNA at a fullerene-C60/GCE
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-04-17T19:26:45Z
dc.journal.volume
69
dc.journal.pagination
369-381
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Gholivand, Mohammad Bagher. Razi University. Faculty of Chemistry; Irán
dc.description.fil
Fil: Jalalvand, Ali R.. Razi University. Faculty of Chemistry; Irán
dc.description.fil
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Cátedra de Química Analítica I. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina
dc.journal.title
International Journal Of Biological Macromolecules
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ijbiomac.2014.05.074
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S014181301400381X
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