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Artículo

Superior mesenteric ganglion neural modulation of ovarian angiogenesis, apoptosis and proliferation by the neuroactive steroid allopregnanolone

Cáceres Gimenez, Antonella Rosario RamonaIcon ; Campo Verde Arbocco, FiorellaIcon ; Cardone, Daniela Alejandra; Sanhueza, María de Los ÁngelesIcon ; Casais, MarilinaIcon ; Vega Orozco, Adriana SoledadIcon ; Laconi, Myriam RaquelIcon
Fecha de publicación: 10/2021
Editorial: Wiley Blackwell Publishing, Inc
Revista: Journal of Neuroendocrinology
ISSN: 0953-8194
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Reproductiva

Resumen

Allopregnanolone (ALLO), a potent neuroactive steroid, is synthesized and active in the peripheral nervous system. Previous studies have shown that ALLO participates in the central regulation of reproduction with effects on ovarian physiology, although there is little evidence for its ability to modulate peripheral tissues. The present study aimed to determine whether ALLO, administered to an ex vivo system that comprises the superior mesenteric ganglion (SMG), the ovarian nervous plexus (ONP) and the ovary (O), or to the denervated ovary (DO), was able to modify ovarian apoptosis, proliferation and angiogenesis. For this purpose, the SMG-ONP-O system and DO were incubated during 120 min at 37°C, in the presence of two ALLO doses (0.06 µm and 6 µm). The intrinsic and extrinsic pathways of apoptosis were analyzed. Incubation of the SMG-ONP-O system with ALLO 0.06 µm led to an increase in the BAX/BCL-2 ratio and a reduction of FAS-L mRNA levels. ALLO 6 µm induced a decrease of FAS-L levels. Incubation of DO with ALLO 0.06 µm reduced FAS-L, whereas ALLO 6 µm significantly increased it. Cyclin D1 mRNA was measured to evaluate proliferation. Treatment with ALLO 6 µm increased proliferation in both SMG-ONP-O and DO. ALLO 0.06 µm produced an increase of Cyclin D1 in DO only. Administration of either ALLO dose led to a higher ovarian expression of vascular endothelial growth factor in the SMG-ONP-O system, but a lower one in the DO system. ALLO 6 µm induced ovarian sensitization to GABA by increasing GABAA receptor expression. In conclusion, ALLO participates in the peripheral neural modulation of ovarian physiology. It can also interact directly with the ovarian tissue, modulating key mechanisms involved in normal and pathological processes in a dose-dependent manner.
Palabras clave: ALLOPREGNANOLONE , GABAAR , NEUROACTIVE STEROID , OVARY , PNS
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/153356
URL: https://onlinelibrary.wiley.com/doi/10.1111/jne.13056
DOI: http://dx.doi.org/10.1111/jne.13056
Colecciones
Articulos(IMBECU)
Articulos de INST. DE MEDICINA Y BIO. EXP. DE CUYO
Citación
Cáceres Gimenez, Antonella Rosario Ramona; Campo Verde Arbocco, Fiorella; Cardone, Daniela Alejandra; Sanhueza, María de Los Ángeles; Casais, Marilina; et al.; Superior mesenteric ganglion neural modulation of ovarian angiogenesis, apoptosis and proliferation by the neuroactive steroid allopregnanolone; Wiley Blackwell Publishing, Inc; Journal of Neuroendocrinology; 34; 2; 10-2021; 1-15
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