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dc.contributor.author
Castillo, Ana Fernanda
dc.contributor.author
Orlando, Ulises Daniel
dc.contributor.author
Lopez, Paula Veronica
dc.contributor.author
Solano, Angela Rosario
dc.contributor.author
Maloberti, Paula Mariana
dc.contributor.author
Podesta, Ernesto Jorge
dc.date.available
2017-04-17T17:41:58Z
dc.date.issued
2015-11
dc.identifier.citation
Castillo, Ana Fernanda; Orlando, Ulises Daniel; Lopez, Paula Veronica; Solano, Angela Rosario; Maloberti, Paula Mariana; et al.; Gene expression profile and signaling pathways in MCF-7 breast cancer cells mediated by acyl-CoA synthetase 4 overexpression; OMICS International; Transcriptomics; 3; 2; 11-2015; 1-26
dc.identifier.issn
2329-8936
dc.identifier.uri
http://hdl.handle.net/11336/15298
dc.description.abstract
Aim: Breast cancer comprises a heterogeneous group of diseases that vary in morphology, biology, behavior and response to therapy. Previous studies have identified an acyl-CoA synthetase 4 (ACSL4) gene-expression pattern correlated with very aggressive tumors. In particular, we have used the tetracycline Tet-Off system to stably transfect non-aggressive breast cancer MCF-7 cells and developed a stable line overexpressing ACSL4 (MCF-7 Tet-Off/ACSL4). As a result, we have proven that cell transfection solely with ACSL4 cDNA renders a highly aggressive phenotype in vitro and results in the development of growing tumors when injected into nude mice. Nevertheless, and in spite of widespread consensus on the role of ACSL4 in mediating an aggressive phenotype in breast cancer, the early steps through which ACSL4 increases tumor growth and progression have been scarcely described and need further elucidation. For this reason, the goal of this work was to study the gene expression profile and the signaling pathways triggered by ACSL4 overexpression in the mechanism that leads to an aggressive phenotype in breast cancer. Methods: We have performed a massive in-depth mRNA sequencing approach and a reverse-phase protein array using MCF-7 Tet-Off/ACSL4 cells as a model to identify gene expression and functional proteomic signatures specific to ACSL4 overexpression. Results and Conclusion: The sole expression of ACSL4 displays a distinctive transcriptome and functional proteomic profile. Furthermore, gene networks most significantly upregulated in breast cancer cells overexpressing ACSL4 are associated to the regulation of embryonic and tissue development, cellular movement and DNA replication and repair. In conclusion, ACSL4 is an upstream regulator of tumorigenic pathways. Because an aggressive tumor phenotype appears in the early stages of metastatic progression, the previously unknown mediators of ACSL4 might.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
OMICS International
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Acyl-Coa Synthetase 4
dc.subject
Breast Cancer
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Functional Proteomics
dc.subject
Transcriptome
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Gene Signature
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Gene expression profile and signaling pathways in MCF-7 breast cancer cells mediated by acyl-CoA synthetase 4 overexpression
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-04-07T14:29:03Z
dc.journal.volume
3
dc.journal.number
2
dc.journal.pagination
1-26
dc.journal.pais
India
dc.journal.ciudad
Hyderabad
dc.description.fil
Fil: Castillo, Ana Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquimica; Argentina
dc.description.fil
Fil: Orlando, Ulises Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquimica; Argentina
dc.description.fil
Fil: Lopez, Paula Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquimica; Argentina
dc.description.fil
Fil: Solano, Angela Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquimica; Argentina
dc.description.fil
Fil: Maloberti, Paula Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquimica; Argentina
dc.description.fil
Fil: Podesta, Ernesto Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquimica; Argentina
dc.journal.title
Transcriptomics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.esciencecentral.org/journals/gene-expression-profile-and-signaling-pathways-in-mcf7-breast-cancercells-mediated-by-acylcoa-synthetase-4-overexpression-2329-8936-1000120.php?aid=65696
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4172/2329-8936.1000120


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