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dc.contributor.author
Silberstein, Claudia Marcela
dc.contributor.author
Copeland, Diane P
dc.contributor.author
Chiang, Wei Lien
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Repetto, Horacio A.
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Ibarra, Cristina Adriana
dc.date.available
2022-03-07T12:18:32Z
dc.date.issued
2008-12
dc.identifier.citation
Silberstein, Claudia Marcela; Copeland, Diane P; Chiang, Wei Lien; Repetto, Horacio A.; Ibarra, Cristina Adriana; A glucosylceramide synthase inhibitor prevents the cytotoxic effects of Shiga toxin-2 on human renal tubular epithelial cells; Bentham Science Publishers; Journal of Epithelial Biology and Pharmacology; 1; 12-2008; 71-75
dc.identifier.issn
1875-0443
dc.identifier.uri
http://hdl.handle.net/11336/152947
dc.description.abstract
Shiga toxin-2 binds to the globotriaosyl-ceramide receptor on the plasma membrane of target cells. The highlevel expression of this receptor in renal epithelial cells may account, at least in part, for acute renal failure observed inchildren with hemolytic uremic syndrome. The cytotoxic effect of Shiga toxin-2 was assayed on primary cultures of humanrenal tubular epithelial cells treated with a new specific inhibitor of glucosylceramide synthase (C-9), the ratelimitingfirst step in the glycosphingolipid biosynthetic pathway. The treatment of the cells with 1-5 M C-9 for at least24 h significantly neutralized the action of 1 ng/ml Shiga toxin-2 on cell viability. The expression levels of globotriaosylceramidesignificantly decreased when cells were incubated with 1 M C-9 for 48 h. We propose here that prevention ofglobotriaosyl-ceramide synthesis by the C-9 could be a novel substrate inhibition therapy to neutralize Shiga toxin-2 actionin renal epithelial cells.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Bentham Science Publishers
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Shiga toxin
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proximal tubule epithelial cells
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globotriaosyl-ceramide
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glucosylceramide synthase
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Enfermedades Infecciosas
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Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
A glucosylceramide synthase inhibitor prevents the cytotoxic effects of Shiga toxin-2 on human renal tubular epithelial cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-12-03T20:49:06Z
dc.journal.volume
1
dc.journal.pagination
71-75
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Silberstein, Claudia Marcela. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
dc.description.fil
Fil: Copeland, Diane P. No especifíca;
dc.description.fil
Fil: Chiang, Wei Lien. No especifíca;
dc.description.fil
Fil: Repetto, Horacio A.. Hospital Nacional Profesor Alejandro Posadas; Argentina
dc.description.fil
Fil: Ibarra, Cristina Adriana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
dc.journal.title
Journal of Epithelial Biology and Pharmacology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://benthamopen.com/contents/pdf/JEBP/JEBP-1-71.pdf
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