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dc.contributor.author
Abrahamyan, Levon G.
dc.contributor.author
Chatel Chaix, Laurent
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Ajamian, Lara
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Milev, Miroslav P.
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Monette, Anne
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Clément, Jean François
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Song, Rujun
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Lehmann, Martin
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DesGroseillers, Luc
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Laughrea, Michael
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Boccaccio, Graciela Lidia
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Mouland, Andrew J.
dc.date.available
2017-04-17T14:53:27Z
dc.date.issued
2010-02
dc.identifier.citation
Abrahamyan, Levon G.; Chatel Chaix, Laurent; Ajamian, Lara; Milev, Miroslav P.; Monette, Anne; et al.; Novel Staufen1 ribonucleoproteins prevent formation of stress granules but favour encapsidation of HIV-1 genomic RNA; Company Of Biologists; Journal Of Cell Science; 123; Pte 3; 2-2010; 369-383
dc.identifier.uri
http://hdl.handle.net/11336/15285
dc.description.abstract
Human immunodeficiency virus type 1 (HIV-1) Gag selects for and mediates genomic RNA (vRNA) encapsidation into progeny virus particles. The host protein, Staufen1 interacts directly with Gag and is found in ribonucleoprotein (RNP) complexes containing vRNA, which provides evidence that Staufen1 plays a role in vRNA selection and encapsidation. In this work, we show that Staufen1, vRNA and Gag are found in the same RNP complex. These cellular and viral factors also colocalize in cells and constitute novel Staufen1 RNPs (SHRNPs) whose assembly is strictly dependent on HIV-1 expression. SHRNPs are distinct from stress granules and processing bodies, are preferentially formed during oxidative stress and are found to be in equilibrium with translating polysomes. Moreover, SHRNPs are stable, and the association between Staufen1 and vRNA was found to be evident in these and other types of RNPs. We demonstrate that following Staufen1 depletion, apparent supraphysiologic-sized SHRNP foci are formed in the cytoplasm and in which Gag, vRNA and the residual Staufen1 accumulate. The depletion of Staufen1 resulted in reduced Gag levels and deregulated the assembly of newly synthesized virions, which were found to contain several-fold increases in vRNA, Staufen1 and other cellular proteins. This work provides new evidence that Staufen1-containing HIV-1 RNPs preferentially form over other cellular silencing foci and are involved in assembly, localization and encapsidation of vRNA.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Company Of Biologists
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Hiv
dc.subject
Staufen
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Stress Granule
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Capside
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Novel Staufen1 ribonucleoproteins prevent formation of stress granules but favour encapsidation of HIV-1 genomic RNA
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-02-07T13:52:52Z
dc.identifier.eissn
1477-9137
dc.journal.volume
123
dc.journal.number
Pte 3
dc.journal.pagination
369-383
dc.journal.pais
Reino Unido
dc.description.fil
Fil: Abrahamyan, Levon G.. Davis Jewish General Hospital; Canadá
dc.description.fil
Fil: Chatel Chaix, Laurent. Davis Jewish General Hospital; Canadá
dc.description.fil
Fil: Ajamian, Lara. Mc Gill University; Canadá. Davis Jewish General Hospital; Canadá
dc.description.fil
Fil: Milev, Miroslav P.. Mc Gill University; Canadá. Davis Jewish General Hospital; Canadá
dc.description.fil
Fil: Monette, Anne. Mc Gill University; Canadá. Davis Jewish General Hospital; Canadá
dc.description.fil
Fil: Clément, Jean François. Davis Jewish General Hospital; Canadá
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Fil: Song, Rujun. Mc Gill University; Canadá. Davis Jewish General Hospital; Canadá
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Fil: Lehmann, Martin. Davis Jewish General Hospital; Canadá
dc.description.fil
Fil: DesGroseillers, Luc. University Of Montreal; Canadá
dc.description.fil
Fil: Laughrea, Michael. Mc Gill University; Canadá. Davis Jewish General Hospital; Canadá
dc.description.fil
Fil: Boccaccio, Graciela Lidia. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
dc.description.fil
Fil: Mouland, Andrew J.. Mc Gill University; Canadá. Davis Jewish General Hospital; Canadá
dc.journal.title
Journal Of Cell Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://jcs.biologists.org/content/123/3/369.long
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1242/jcs.055897
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