Artículo
The Impact of IFN-g; Receptor on SLPI Expression in Active Tuberculosis: Association with Disease Severity
Tateosian, Nancy Liliana
; Pasquinelli, Virginia
; Hernández Del Pino, Rodrigo Emanuel; Ambrosi, Nella Gabriela
; Guerrieri, Diego
; Pedraza Sánchez, Sigifredo; Santucci, Natalia Estefanía
; D'attilio, Luciano David
; Pellegrini, Joaquín Miguel
; Araujo Solis, María A.; Musella, Rosa M.; Palmero, Domingo J.; Hernandez Pando, Rogelio; Garcia, Veronica Edith
; Chuluyan, Hector Eduardo
Fecha de publicación:
05/2014
Editorial:
Amer Soc Investigative Pathology, Inc
Revista:
American Journal Of Pathology
ISSN:
0002-9440
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Interferon (IFN)-g displays a critical role in tuberculosis (TB), modulating the innate and adaptive immune responses. Previously, we reported that secretory leukocyte protease inhibitor (SLPI) is a pattern recognition receptor with anti-mycobacterial activity against Mycobacterium tuberculosis (Mtb). Herein, we determined whether IFN-g modulated the levels of SLPI in TB patients. Plasma levels of SLPI and IFN-g were studied in healthy donors (HDs) and TB patients. Peripheral blood mononuclear cells from HDs and patients with TB or defective IFN-g receptor 1* were stimulated with Mtb antigen and SLPI, and IFN-gR expression levels were measured. Both SLPI and IFN-g were significantly enhanced in plasma from those with TB compared with HDs. A direct association between SLPI levels and the severity of TB was detected. In addition, Mtb antigen stimulation decreased the SLPI produced by peripheral blood mononuclear cells from HDs, but not from TB or IFN-gR patients. Neutralization of IFN-g reversed the inhibition of SLPI induced by Mtb antigen in HDs, but not in TB patients. Furthermore, recombinant IFN-g was unable to modify the expression of SLPI in TB patients. Finally, IFN-gR expression was lower in TB compared with HD peripheral blood mononuclear cells. These results show that Mtb-induced IFN-g down-modulated SLPI levels by signaling through the IFNgR in HDs. This inhibitory mechanism was not observed in TB, probably because of the low expression of IFN-gR detected in these individuals. (Am J Pathol 2014, 184: 1e6
Palabras clave:
Tuberculosis
,
Ifn-G
,
Slpi
,
Receptor
Archivos asociados
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Colecciones
Articulos(CCT - ROSARIO)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Articulos(CEFYBO)
Articulos de CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Articulos de CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Articulos(OCA CIUDAD UNIVERSITARIA)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA CIUDAD UNIVERSITARIA
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA CIUDAD UNIVERSITARIA
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Tateosian, Nancy Liliana; Pasquinelli, Virginia; Hernández Del Pino, Rodrigo Emanuel; Ambrosi, Nella Gabriela; Guerrieri, Diego; et al.; The Impact of IFN-g; Receptor on SLPI Expression in Active Tuberculosis: Association with Disease Severity; Amer Soc Investigative Pathology, Inc; American Journal Of Pathology; 184; 5; 5-2014; 1268–1273
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