Artículo
A GRF–GIF chimeric protein improves the regeneration efficiency of transgenic plants
Debernardi, Juan Manuel
; Tricoli, David M.; Ercoli, María Florencia
; Hayta, Sadiye; Ronald, Pamela; Palatnik, Javier Fernando
; Dubcovsky, Jorge
Fecha de publicación:
11/2020
Editorial:
Nature Publishing Group
Revista:
Nature Biotechnology
ISSN:
1087-0156
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The potential of genome editing to improve the agronomic performance of crops is often limited by low plant regeneration efficiencies and few transformable genotypes. Here, we show that expression of a fusion protein combining wheat GROWTH-REGULATING FACTOR 4 (GRF4) and its cofactor GRF-INTERACTING FACTOR 1 (GIF1) substantially increases the efficiency and speed of regeneration in wheat, triticale and rice and increases the number of transformable wheat genotypes. GRF4–GIF1 transgenic plants were fertile and without obvious developmental defects. Moreover, GRF4–GIF1 induced efficient wheat regeneration in the absence of exogenous cytokinins, which facilitates selection of transgenic plants without selectable markers. We also combined GRF4–GIF1 with CRISPR–Cas9 genome editing and generated 30 edited wheat plants with disruptions in the gene Q (AP2L-A5). Finally, we show that a dicot GRF–GIF chimera improves regeneration efficiency in citrus, suggesting that this strategy can be applied to dicot crops.
Palabras clave:
GRF
,
GIF
,
regeneracion trigo
,
edicion genica
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Citación
Debernardi, Juan Manuel; Tricoli, David M.; Ercoli, María Florencia; Hayta, Sadiye; Ronald, Pamela; et al.; A GRF–GIF chimeric protein improves the regeneration efficiency of transgenic plants; Nature Publishing Group; Nature Biotechnology; 38; 11; 11-2020; 1274-1279
Compartir
Altmétricas