Artículo
Omp19 enables Brucella abortus to evade the antimicrobial activity from host's proteolytic defense system
Pasquevich, Karina Alejandra
; Carabajal, Marianela Veronica
; Guaimas, Francisco Fernando
; Bruno, Laura Alejandra; Roset, Mara Sabrina
; Coria, Mirta Lorena
; Rey Serrantes, Diego A.; Comerci, Diego José
; Cassataro, Juliana
Fecha de publicación:
06/2019
Editorial:
Frontiers Media
Revista:
Frontiers in Immunology
ISSN:
1664-3224
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Pathogenic microorganisms confront several proteolytic events in the molecular interplay with their host, highlighting that proteolysis and its regulation play an important role during infection. Microbial inhibitors, along with their target endogenous/exogenous enzymes, may directly affect the host’s defense mechanisms and promote infection. Omp19 is a Brucella spp. conserved lipoprotein anchored by the lipid portion in the Brucella outer membrane. Previous work demonstrated that purified unlipidated Omp19 (U-Omp19) has protease inhibitor activity against gastrointestinal and lysosomal proteases. In this work, we found that a Brucella omp19 deletion mutant is highly attenuated in mice when infecting by the oral route. This attenuation can be explained by bacterial increased susceptibility to host proteases met by the bacteria during establishment of infection. Omp19 deletion mutant has a cell division defect when exposed to pancreatic proteases that is linked to cell-cycle arrest in G1-phase, Omp25 degradation on the cell envelope and CtrA accumulation. Moreover, Omp19 deletion mutant is more susceptible to killing by macrophage derived microsomes than wt strain. Preincubation with gastrointestinal proteases led to an increased susceptibility of Omp19 deletion mutant to macrophage intracellular killing. Thus, in this work, we describe for the first time a physiological function of B. abortus Omp19. This activity enables Brucella to better thrive in the harsh gastrointestinal tract, where protection from proteolytic degradation can be a matter of life or death, and afterwards invade the host and bypass intracellular proteases to establish the chronic infection.
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Articulos (IIBIO)
Articulos de INSTITUTO DE INVESTIGACIONES BIOTECNOLOGICAS
Articulos de INSTITUTO DE INVESTIGACIONES BIOTECNOLOGICAS
Citación
Pasquevich, Karina Alejandra; Carabajal, Marianela Veronica; Guaimas, Francisco Fernando; Bruno, Laura Alejandra; Roset, Mara Sabrina; et al.; Omp19 enables Brucella abortus to evade the antimicrobial activity from host's proteolytic defense system; Frontiers Media; Frontiers in Immunology; 10; 1436; 6-2019; 1-14
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