Mostrar el registro sencillo del ítem

dc.contributor.author
Ferraris, Maria Jimena  
dc.contributor.author
Boutillon, Florence  
dc.contributor.author
Bernadet, Marie  
dc.contributor.author
Seilicovich, Adriana  
dc.contributor.author
Goffin, Vincent  
dc.contributor.author
Pisera, Daniel Alberto  
dc.date.available
2017-04-12T19:29:19Z  
dc.date.issued
2012-01  
dc.identifier.citation
Ferraris, Maria Jimena; Boutillon, Florence; Bernadet, Marie; Seilicovich, Adriana; Goffin, Vincent; et al.; Prolactin receptor antagonism in mouse anterior pituitary: effects on cell turnover and prolactin receptor expression; American Physiological Society; American Journal Of Physiology-endocrinology And Metabolism; 302; 3; 1-2012; 356-364  
dc.identifier.issn
0193-1849  
dc.identifier.uri
http://hdl.handle.net/11336/15234  
dc.description.abstract
Since anterior pituitary expresses prolactin receptors, prolactin secreted by lactotropes could exert autocrine or paracrine actions on anterior pituitary cells. In fact, it has been observed that prolactin inhibits its own expression by lactotropes. Our hypothesis is that prolactin participates in the control of anterior pituitary cell turnover. In the present study, we explored the action of prolactin on proliferation and apoptosis of anterior pituitary cells and its effect on the expression of the prolactin receptor. To determine the activity of endogenous prolactin, we evaluated the effect of the competitive prolactin receptor antagonist Δ1–9-G129R-hPRL in vivo, using transgenic mice that constitutively and systemically express this antagonist. The weight of the pituitary gland and the anterior pituitary proliferation index, determined by BrdU incorporation, were higher in transgenic mice expressing the antagonist than in wild-type littermates. In addition, blockade of prolactin receptor in vitro by Δ1–9-G129R-hPRL increased proliferation and inhibited apoptosis of somatolactotrope GH3 cells and of primary cultures of male rat anterior pituitary cells, including lactotropes. These results suggest that prolactin acts as an autocrine/paracrine antiproliferative and proapoptotic factor in the anterior pituitary gland. In addition, anterior pituitary expression of the long isoform of the prolactin receptor, measured by real-time PCR, increased about 10-fold in transgenic mice expressing the prolactin receptor antagonist, whereas only a modest increase in the S3 short-isoform expression was observed. These results suggest that endogenous prolactin may regulate its own biological actions in the anterior pituitary by inhibiting the expression of the long isoform of the prolactin receptor. In conclusion, our observations suggest that prolactin is involved in the maintenance of physiological cell renewal in the anterior pituitary. Alterations in this physiological role of prolactin could contribute to pituitary tumor development.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
American Physiological Society  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Apoptosis  
dc.subject
Antiproliferative Effect  
dc.subject
∆1-9-G129-Hprl  
dc.subject
Long Prolactin Receptor  
dc.subject
Short Prolactin Receptor  
dc.subject
Prolactin Receptor Isoform  
dc.subject.classification
Fisiología  
dc.subject.classification
Medicina Básica  
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Prolactin receptor antagonism in mouse anterior pituitary: effects on cell turnover and prolactin receptor expression  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2017-04-07T14:27:35Z  
dc.identifier.eissn
1522-1555  
dc.journal.volume
302  
dc.journal.number
3  
dc.journal.pagination
356-364  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Bethesda  
dc.description.fil
Fil: Ferraris, Maria Jimena. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Boutillon, Florence. Universite de Paris V; Francia  
dc.description.fil
Fil: Bernadet, Marie. Universite de Paris V; Francia  
dc.description.fil
Fil: Seilicovich, Adriana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Goffin, Vincent. Universite de Paris V; Francia  
dc.description.fil
Fil: Pisera, Daniel Alberto. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Biología Celular e Histología. Centro de Investigación en Reproducción; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.journal.title
American Journal Of Physiology-endocrinology And Metabolism  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://ajpendo.physiology.org/content/302/3/E356.full.html  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1152/ajpendo.00333.2011