Paraboeremia yungensis sp. nov., a new fungal species isolated from Las Yungas, South America, with promising tyrosinase production potential

Abstract

In the context of a bioprospection programme for tyrosinase/L-DOPA- and melanin-producing fungal strains forbiotechnological purposes, a hyperproducer isolate was obtained from Las Yungas rainforest, a relevant biodiverse ecoregionin North-Western Argentina. The selected strain was preliminarily identified as Paraboeremia sp. This is, to the best of ourknowledge, the first native reported species of this genus in South America. Single-gene and multi-locus analyses of theinternal transcribed spacer nuclear ribosomal RNA gene region (ITS), partial large subunit 28S nrDNA region (LSU), RNApolymerase II region (RPB2) and partial β-tubulin gene (TUB2) alignments were carried out to define the phylogeneticidentity of this strain. As part of a polyphasic identification approach, these results were combined with morphologicalstudies of active cultures growing on malt extract, oatmeal and potato dextrose agar plates. Incubation was performedunder diverse conditions to stimulate sporulation for the subsequent micromorphological analysis. Microphotographs ofpycnidia and conidia were taken with a scanning electron microscope. Maximum likelihood and Bayesian Inference analysessupported the location of the strain within the genus Paraboeremia, whilst morphological features allowed distinguishing itfrom previously described species within this genus. Based on the results herein reported, the new South-American speciesParaboeremia yungensis is described and proposed.