Artículo

New tools for recombinant protein production in Escherichia coli : A 5‐year update

Rosano, German LeandroIcon ; Morales, Enrique SalvadorIcon ; Ceccarelli, Eduardo AugustoIcon
Fecha de publicación: 07/2019
Editorial: John Wiley & Sons Inc
Revista: Protein Science
ISSN: 0961-8368
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:

Resumen

The production of proteins in sufficient amounts is key for their study or use as biotherapeutic agents. Escherichia coli is the host of choice for recombinant protein production given its fast growth, easy manipulation, and cost-effectiveness. As such, its protein production capabilities are continuously being improved. Also, the associated tools (such as plasmids and cultivation conditions) are subject of ongoing research to optimize product yield. In this work, we review the latest advances in recombinant protein production in E. coli.
Palabras clave: AFFINITY TAGS , E. COLI STRAINS , ESCHERICHIA COLI , EXPRESSION PLASMID , FUSION PARTNERS , INCLUSION BODIES , RECOMBINANT PROTEIN PRODUCTION
 
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/151837
URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/pro.3668
DOI: http://dx.doi.org/10.1002/pro.3668
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Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Citación
Rosano, German Leandro; Morales, Enrique Salvador; Ceccarelli, Eduardo Augusto; New tools for recombinant protein production in Escherichia coli : A 5‐year update; John Wiley & Sons Inc; Protein Science; 28; 8; 7-2019; 1412-1422
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