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dc.contributor.author
Bañuelos Vazquez, Luis Alfredo
dc.contributor.author
Torres Tejerizo, Gonzalo Arturo
dc.contributor.author
Cervantes de La Luz, Laura
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Girard, Lourdes
dc.contributor.author
Romero, David
dc.contributor.author
Brom, Susana
dc.date.available
2022-02-11T02:46:03Z
dc.date.issued
2019-05
dc.identifier.citation
Bañuelos Vazquez, Luis Alfredo; Torres Tejerizo, Gonzalo Arturo; Cervantes de La Luz, Laura; Girard, Lourdes; Romero, David; et al.; Conjugative transfer between Rhizobium etli endosymbionts inside the root nodule; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 21; 9; 5-2019; 3430-3441
dc.identifier.issn
1462-2912
dc.identifier.uri
http://hdl.handle.net/11336/151829
dc.description.abstract
Since the discovery that biological nitrogen fixation ensues in nodules resulting from the interaction of rhizobia with legumes, nodules were thought to be exclusive for hosting nitrogen-fixing and plant growth promoting bacteria. In this work, we uncover a novel function of nodules, as a niche permissive to acquisition of plasmids via conjugative transfer. We used Rhizobium etli CFN42, which nodulates Phaseolus vulgaris. The genome of R. etli CFN42 contains a chromosome and six plasmids. pRet42a is a conjugative plasmid regulated by Quorum-Sensing (QS), and pRet42d is the symbiotic plasmid. Here, using confocal microscopy and flow cytometry, we show that pRet42a transfers on the root's surface, and unexpectedly, inside the nodules. Conjugation still took place inside nodules, even when it was restricted on the plant surface by placing the QS traI regulator under the promoter of the nitrogenase gene, which is only expressed inside the nodules, or by inhibiting the QS transcriptional induction of transfer genes with a traM antiactivator on an unstable vector maintained on the plant surface and lost inside the nodules. These results conclusively confirm the occurrence of conjugation in these structures, defining them as a protected environment for bacterial diversification.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
RHIZOBIUM
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PLASMIDS
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Conjugative transfer between Rhizobium etli endosymbionts inside the root nodule
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-11-25T16:45:29Z
dc.journal.volume
21
dc.journal.number
9
dc.journal.pagination
3430-3441
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Bañuelos Vazquez, Luis Alfredo. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Cervantes de La Luz, Laura. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Girard, Lourdes. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Romero, David. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Brom, Susana. Universidad Nacional Autónoma de México; México
dc.journal.title
Environmental Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.14645
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/1462-2920.14645
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