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dc.contributor.author
Corvalán, Natalia Andrea
dc.contributor.author
Kembro, Jackelyn Melissa
dc.contributor.author
Clop, Pedro Diego
dc.contributor.author
Perillo, Maria Angelica
dc.date.available
2015-07-27T15:56:53Z
dc.date.issued
2013-08
dc.identifier.citation
Corvalán, Natalia Andrea; Kembro, Jackelyn Melissa; Clop, Pedro Diego; Perillo, Maria Angelica; Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1828; 8; 8-2013; 1754-1764
dc.identifier.issn
0005-2736
dc.identifier.uri
http://hdl.handle.net/11336/1517
dc.description.abstract
The present paper was aimed at evaluating the effect of cholesterol (CHO) on the voltage-induced lipid 22 Q5 pore formation in bilayer membranes through a global characterization of the temporal dynamics of 23 the fluctuation pattern of ion currents. The bilayer model used was black lipid membranes (BLMs) of 24 Q6 palmitoyloleoylphosphatidylethanolamine and palmitoyloleoylphosphatidylcholine (POPE:POPC) at a 7:3 25 molar ratio in the absence (BLM0) or in the presence of 30 (BLM30), 40 (BLM40) or 50 (BLM50) mol% of cholesterol 26 with respect to total phospholipids. Electrical current intensities (I)weremeasured in voltage (ΔV) clamped condi- 27 tions at ΔV ranging between 0 and ±200 mV. The autocorrelation parameter α derived from detrended fluc- 28 tuation analysis (DFA) on temporal fluctuation patterns of electrical currents allowed discriminating 29 between non-correlated (α = 0.5, white noise) and long-range correlated (0.5 b α b 1) behaviors. The in- 30 crease in |ΔV| as well as in cholesterol content increased the number of conductance states, the magnitude of con- 31 ductance level, the capacitance of the bilayers and increased the tendency towards the development of long-range 32 autocorrelated (fractal) processes (0.5 b α b 1) in lipid channel generation. Experimentswere performed above the 33 phase transition temperature of the lipidmixtures, but compositions used predicted a superlattice-like organization. 34 This leads to the conclusion that structural defects other than phase coexistencemay promote lipid channel forma- 35 tion under voltage clamped conditions. Furthermore, cholesterol controls the voltage threshold that allows the per- 36 colation of channel behavior where isolated channels become an interconnected network.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BLACK LIPID MEMBRANES
dc.subject
CHOLESTEROL
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DETRENDED FLUCTUATION ANALYSIS
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IONIC CURRENT
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LIPID PORES
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Biofísica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Cholesterol favors the emergence of a long-range autocorrelated fluctuation pattern in voltage-induced ionic currents through lipid bilayers
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
1828
dc.journal.number
8
dc.journal.pagination
1754-1764
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Corvalán, Natalia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
dc.description.fil
Fil: Kembro, Jackelyn Melissa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
dc.description.fil
Fil: Clop, Pedro Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
dc.description.fil
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
dc.journal.title
Biochimica et Biophysica Acta - Biomembranes
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbamem.2013.03.019
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