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dc.contributor.author
Montemurro, Milagros
dc.contributor.author
Schwaighofer, Andreas
dc.contributor.author
Schmidt, Anatol
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Culzoni, Maria Julia
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Mayer, Helmut K.
dc.contributor.author
Lendl, Bernhard
dc.date.available
2022-02-07T18:33:38Z
dc.date.issued
2019-08
dc.identifier.citation
Montemurro, Milagros; Schwaighofer, Andreas; Schmidt, Anatol; Culzoni, Maria Julia; Mayer, Helmut K.; et al.; High-throughput quantitation of bovine milk proteins and discrimination of commercial milk types by external cavity-quantum cascade laser spectroscopy and chemometrics; Royal Society of Chemistry; Analyst; 144; 18; 8-2019; 5571-5579
dc.identifier.issn
0003-2654
dc.identifier.uri
http://hdl.handle.net/11336/151483
dc.description.abstract
Analysis of bovine milk proteins is crucial in many food and non-food industrial applications, nevertheless labour-intensive wet-chemical, low-throughput methods are still routinely used. In this work, external cavity-quantum cascade laser (EC-QCL) mid-infrared spectroscopy is employed as a rapid method for protein analysis of commercial bovine milk. Combined analysis of the amide I and II bands enabled quantitation of individual proteins (casein, β-lactoglobulin, α-lactalbumin) and total protein content. IR spectra of spiked and diluted milk samples were employed for calibration of the target analytes in the presence of a complex matrix by partial least squares (PLS) regression modelling. A sample set of different milk types (pasteurized; differently processed extended shelf life, ESL; ultra-high temperature, UHT) was analysed, and results agreed well with reference methods. Quantitation of temperature sensitive proteins enables detailed distinction between milk types experiencing different heat loads during processing, and discrimination between diverse bovine milk types is successfully demonstrated.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Royal Society of Chemistry
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.subject
MID-IR SPECTROSCOPY
dc.subject
MILK
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CHEMOMETRICS
dc.subject.classification
Química Analítica
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
High-throughput quantitation of bovine milk proteins and discrimination of commercial milk types by external cavity-quantum cascade laser spectroscopy and chemometrics
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-11-27T18:48:03Z
dc.journal.volume
144
dc.journal.number
18
dc.journal.pagination
5571-5579
dc.journal.pais
Reino Unido
dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Montemurro, Milagros. Vienna University of Technology; Austria. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Schwaighofer, Andreas. Vienna University of Technology; Austria
dc.description.fil
Fil: Schmidt, Anatol. University of Natural Resources and Life Sciences; Austria
dc.description.fil
Fil: Culzoni, Maria Julia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Mayer, Helmut K.. University of Natural Resources and Life Sciences; Austria
dc.description.fil
Fil: Lendl, Bernhard. Vienna University of Technology; Austria
dc.journal.title
Analyst
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://xlink.rsc.org/?DOI=C9AN00746F
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1039/C9AN00746F
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