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Artículo

Detection of MicroRNA processing intermediates through RNA ligation approaches

Moro, BelénIcon ; Rojas, Arantxa Maria LarisaIcon ; Palatnik, Javier FernandoIcon
Fecha de publicación: 03/2019
Editorial: Humana Press
Revista: Methods in Molecular Biology
ISSN: 1064-3745
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

MicroRNAs (miRNA) are small RNAs of 20–22 nt that regulate diverse biological pathways through the modulation of gene expression. miRNAs recognize target RNAs by base complementarity and guide them to degradation or translational arrest. They are transcribed as longer precursors with extensive secondary structures. In plants, these precursors are processed by a complex harboring DICER-LIKE1 (DCL1), which cuts on the precursor stem region to release the mature miRNA together with the miRNA*. In both plants and animals, the miRNA precursors contain spatial clues that determine the position of the miRNA along their sequences. DCL1 is assisted by several proteins, such as the double-stranded RNA binding protein, HYPONASTIC LEAVES1 (HYL1), and the zinc finger protein SERRATE (SE). The precise biogenesis of miRNAs is of utter importance since it determines the exact nucleotide sequence of the mature small RNAs and therefore the identity of the target genes. miRNA processing itself can be regulated and therefore can determine the final small RNA levels and activity. Here, we describe methods to analyze miRNA processing intermediates in plants. These approaches can be used in wild-type or mutant plants, as well as in plants grown under different conditions, allowing a molecular characterization of the miRNA biogenesis from the RNA precursor perspective.
Palabras clave: ARABIDOPSIS , MICRORNA , NGS , PLANTS , PRECURSOR , PROCESSING , RNA LIGATION
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/151402
DOI: http://dx.doi.org/10.1007/978-1-4939-9042-9_20
URL: https://link.springer.com/protocol/10.1007%2F978-1-4939-9042-9_20
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Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Citación
Moro, Belén; Rojas, Arantxa Maria Larisa; Palatnik, Javier Fernando; Detection of MicroRNA processing intermediates through RNA ligation approaches; Humana Press; Methods in Molecular Biology; 1932; 3-2019; 261-283
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