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dc.contributor.author
Mendieta, Carolina Monica
dc.contributor.author
Felissia, Fernando Esteban
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Arismendy Pabón, Ana María
dc.contributor.author
Kruyeniski, Julia
dc.contributor.author
Area, Maria Cristina
dc.date.available
2022-02-01T14:49:50Z
dc.date.issued
2021-09
dc.identifier.citation
Mendieta, Carolina Monica; Felissia, Fernando Esteban; Arismendy Pabón, Ana María; Kruyeniski, Julia; Area, Maria Cristina; Enzymatic hydrolysis and fermentation strategies for the biorefining of pine sawdust; North Carolina State University. Department of Wood and Paper Science; Bioresources; 16; 4; 9-2021; 7474-7491
dc.identifier.issn
1930-2126
dc.identifier.uri
http://hdl.handle.net/11336/151070
dc.description.abstract
This work aims to evaluate second-generation bioethanol production from the soda-ethanol pulp of pine sawdust via two strategies: separate hydrolysis and fermentation and simultaneous saccharification and fermentation. A kinetics study of the enzymatic hydrolysis of separate hydrolysis and fermentation was included as a design tool. Three soda-ethanol pulps (with different chemical compositions), Cellic® Ctec2 cellulolytic enzymes, and Saccharomyces cerevisiae IMR 1181 (SC 1181) yeast were employed. The obtained kinetic parameters were as follows: an apparent constant (k) of 11.4 h-1, which represents the link frequency between cellulose and cellulase; a Michaelis-Menten apparent constant (KM) of 23.5 gL-1, that indicates the cellulose/cellulase affinity; and the apparent constant of inhibition between cellulose-glucose and cellulase (KI), which was 2.9 gL-1, 3.1 gL-1, and 6.6 gL-1 for pulps 1, 2, and 3, respectively. The kinetic model was applicable, since the calculated glucose values fit the experimental values. High bioethanol yields were obtained for pulp 3 in the separate hydrolysis and fermentation and simultaneous saccharification and fermentation processes (89.3% and 100% after 13 h and 72 h, respectively).
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
North Carolina State University. Department of Wood and Paper Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BIOREFINERY
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PINE SAWDUST
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BIOETHANOL
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KINETIC MODEL
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MICHAELIS-MENTEN
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Papel y Madera
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Ingeniería de los Materiales
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Enzymatic hydrolysis and fermentation strategies for the biorefining of pine sawdust
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-12-03T20:38:16Z
dc.journal.volume
16
dc.journal.number
4
dc.journal.pagination
7474-7491
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Raleigh
dc.description.fil
Fil: Mendieta, Carolina Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
dc.description.fil
Fil: Felissia, Fernando Esteban. Universidad Nacional de Misiones; Argentina
dc.description.fil
Fil: Arismendy Pabón, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
dc.description.fil
Fil: Kruyeniski, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
dc.description.fil
Fil: Area, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina
dc.journal.title
Bioresources
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_16_4_7474_Mendieta_Enzymatic_Hydrolysis_Fermentation_Strategies
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