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dc.contributor.author
Mendieta, Carolina Monica  
dc.contributor.author
Felissia, Fernando Esteban  
dc.contributor.author
Arismendy Pabón, Ana María  
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Kruyeniski, Julia  
dc.contributor.author
Area, Maria Cristina  
dc.date.available
2022-02-01T14:49:50Z  
dc.date.issued
2021-09  
dc.identifier.citation
Mendieta, Carolina Monica; Felissia, Fernando Esteban; Arismendy Pabón, Ana María; Kruyeniski, Julia; Area, Maria Cristina; Enzymatic hydrolysis and fermentation strategies for the biorefining of pine sawdust; North Carolina State University. Department of Wood and Paper Science; Bioresources; 16; 4; 9-2021; 7474-7491  
dc.identifier.issn
1930-2126  
dc.identifier.uri
http://hdl.handle.net/11336/151070  
dc.description.abstract
This work aims to evaluate second-generation bioethanol production from the soda-ethanol pulp of pine sawdust via two strategies: separate hydrolysis and fermentation and simultaneous saccharification and fermentation. A kinetics study of the enzymatic hydrolysis of separate hydrolysis and fermentation was included as a design tool. Three soda-ethanol pulps (with different chemical compositions), Cellic® Ctec2 cellulolytic enzymes, and Saccharomyces cerevisiae IMR 1181 (SC 1181) yeast were employed. The obtained kinetic parameters were as follows: an apparent constant (k) of 11.4 h-1, which represents the link frequency between cellulose and cellulase; a Michaelis-Menten apparent constant (KM) of 23.5 gL-1, that indicates the cellulose/cellulase affinity; and the apparent constant of inhibition between cellulose-glucose and cellulase (KI), which was 2.9 gL-1, 3.1 gL-1, and 6.6 gL-1 for pulps 1, 2, and 3, respectively. The kinetic model was applicable, since the calculated glucose values fit the experimental values. High bioethanol yields were obtained for pulp 3 in the separate hydrolysis and fermentation and simultaneous saccharification and fermentation processes (89.3% and 100% after 13 h and 72 h, respectively).  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
North Carolina State University. Department of Wood and Paper Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
BIOREFINERY  
dc.subject
PINE SAWDUST  
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BIOETHANOL  
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KINETIC MODEL  
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MICHAELIS-MENTEN  
dc.subject.classification
Papel y Madera  
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Ingeniería de los Materiales  
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INGENIERÍAS Y TECNOLOGÍAS  
dc.title
Enzymatic hydrolysis and fermentation strategies for the biorefining of pine sawdust  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2021-12-03T20:38:16Z  
dc.journal.volume
16  
dc.journal.number
4  
dc.journal.pagination
7474-7491  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Raleigh  
dc.description.fil
Fil: Mendieta, Carolina Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina  
dc.description.fil
Fil: Felissia, Fernando Esteban. Universidad Nacional de Misiones; Argentina  
dc.description.fil
Fil: Arismendy Pabón, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina  
dc.description.fil
Fil: Kruyeniski, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina  
dc.description.fil
Fil: Area, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Materiales de Misiones. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Materiales de Misiones; Argentina  
dc.journal.title
Bioresources  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_16_4_7474_Mendieta_Enzymatic_Hydrolysis_Fermentation_Strategies