Artículo
Role of SPARC in the epithelial-mesenchymal transition induced by PTHrP in human colon cancer cells
Carriere, Pedro Matias
; Calvo, Natalia Graciela
; Novoa Díaz, María Belén
; López Moncada, Fernanda; Herrera, Alexander; Torres, María José; Alonso, Exequiel Gonzalo
; Gandini, Norberto Ariel
; Gigola, Graciela; Contreras, Héctor R.; Gentili, Claudia Rosana
Fecha de publicación:
06/2021
Editorial:
Elsevier Ireland
Revista:
Molecular and Cellular Endocrinology
ISSN:
0303-7207
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Parathyroid hormone-related peptide (PTHrP) exerts its effects on cells derived from colorectal cancer (CRC) and tumor microenvironment and is involved in processes requiring the epithelial-mesenchymal transition (EMT). Here, we report that PTHrP modulates factors expression and morphological changes associated with EMT in HCT116 cells from CRC. PTHrP increased the protein expression of SPARC, a factor involved in EMT, in HCT116 cells but not in Caco-2 cells also from CRC but with less aggressiveness. PTHrP also increased SPARC expression and its subsequent release from endothelial HMEC-1 cells. The conditioned media of PTHrP-treated HMEC-1 cells induced early changes related to EMT in HCT116 cells. Moreover, SPARC treatment on HCT116 cells potentiated PTHrP modulation in E-cadherin expression and cell migration. In vivo PTHrP also increased SPARC expression and decreased E-cadherin expression. These results suggest a novel PTHrP action on CRC progression involving the microenvironment in the modulation of events associated with EMT.
Palabras clave:
COLON CANCER
,
EMT
,
PTHRP
,
SPARC
,
TUMOR MICROENVIRONMENT
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Identificadores
Colecciones
Articulos(INBIOSUR)
Articulos de INSTITUTO DE CIENCIAS BIOLOGICAS Y BIOMEDICAS DEL SUR
Articulos de INSTITUTO DE CIENCIAS BIOLOGICAS Y BIOMEDICAS DEL SUR
Articulos(INIBIBB)
Articulos de INST.DE INVEST.BIOQUIMICAS BAHIA BLANCA (I)
Articulos de INST.DE INVEST.BIOQUIMICAS BAHIA BLANCA (I)
Citación
Carriere, Pedro Matias; Calvo, Natalia Graciela; Novoa Díaz, María Belén; López Moncada, Fernanda; Herrera, Alexander; et al.; Role of SPARC in the epithelial-mesenchymal transition induced by PTHrP in human colon cancer cells; Elsevier Ireland; Molecular and Cellular Endocrinology; 530; 6-2021; 1-13
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