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dc.contributor.author
Xiao, Yao
dc.contributor.author
Amaral, Thiago F.
dc.contributor.author
Ross, Pablo J.
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Soto, Delia A.
dc.contributor.author
Diffenderfer, Kenneth E.
dc.contributor.author
Pankonin, Aimee R.
dc.contributor.author
Jeensuk, Surawich
dc.contributor.author
Tribulo, Paula
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dc.contributor.author
Hansen, Peter J.
dc.date.available
2022-01-26T20:20:53Z
dc.date.issued
2021-04
dc.identifier.citation
Xiao, Yao; Amaral, Thiago F.; Ross, Pablo J.; Soto, Delia A.; Diffenderfer, Kenneth E.; et al.; Importance of WNT-dependent signaling for derivation and maintenance of primed pluripotent bovine embryonic stem cells†; Society for the Study of Reproduction; Biology of Reproduction; 105; 1; 4-2021; 52-63
dc.identifier.issn
0006-3363
dc.identifier.uri
http://hdl.handle.net/11336/150724
dc.description.abstract
The WNT signaling system plays an important but paradoxical role in the regulation of pluripotency. In the cow, IWR-1, which inhibits canonical WNT activation and has WNT-independentactions, promotes the derivation of primed pluripotent embryonic stem cells from the blastocyst.Here, we describe a series of experiments to determine whether derivation of embryonic stem cellscould be generated by replacing IWR-1 with other inhibitors of WNT signaling. Results confirmthe importance of inhibition of canonical WNT signaling for the establishment of pluripotentembryonic stem cells in cattle and indicate that the actions of IWR-1 can be mimicked by theWNT secretion inhibitor IWP2 but not by the tankyrase inhibitor XAV939 or WNT inhibitoryprotein dickkopf 1. The role of Janus kinase-mediated signaling pathways for the maintenanceof pluripotency of embryonic stem cells was also evaluated. Maintenance of pluripotency ofembryonic stem cells lines was blocked by a broad inhibitor of Janus kinase, even though the cellsdid not express phosphorylated signal transducer and activator of transcription 3 (pSTAT3). Furtherstudies with blastocysts indicated that IWR-1 blocks the activation of pSTAT3. A likely explanationis that IWR-1 blocks differentiation of embryonic stem cells into a pSTAT3+ lineage. In conclusion,results presented here indicate the importance of inhibition of WNT signaling for the derivationof pluripotent bovine embryonic stem cells, the role of Janus kinase signaling for maintenance ofpluripotency, and the participation of IWR-1 in the inhibition of activation of STAT3.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Society for the Study of Reproduction
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
WNT
dc.subject
Stem Cell
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Embryo
dc.subject
Bovine
dc.subject.classification
Biología del Desarrollo
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Importance of WNT-dependent signaling for derivation and maintenance of primed pluripotent bovine embryonic stem cells†
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-01-25T14:39:29Z
dc.identifier.eissn
1529-7268
dc.journal.volume
105
dc.journal.number
1
dc.journal.pagination
52-63
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Xiao, Yao. University of Florida; Estados Unidos
dc.description.fil
Fil: Amaral, Thiago F.. University of Florida; Estados Unidos
dc.description.fil
Fil: Ross, Pablo J.. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Soto, Delia A.. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Diffenderfer, Kenneth E.. Stem Cell Core, Salk Institute For Biological Studies; Estados Unidos
dc.description.fil
Fil: Pankonin, Aimee R.. Stem Cell Core, Salk Institute For Biological Studies; Estados Unidos
dc.description.fil
Fil: Jeensuk, Surawich. Stem Cell Core, Salk Institute For Biological Studies; Estados Unidos
dc.description.fil
Fil: Tribulo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Instituto de Reproducción Animal Córdoba; Argentina
dc.description.fil
Fil: Hansen, Peter J.. University of Florida; Estados Unidos
dc.journal.title
Biology of Reproduction
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/advance-article/doi/10.1093/biolre/ioab075/6246304
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/biolre/ioab075
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