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dc.contributor.author
Martínez, Ana Laura
dc.contributor.author
Quiroz, Facundo José
dc.contributor.author
Carrera, Alicia Delia
dc.date.available
2022-01-25T18:55:14Z
dc.date.issued
2021-09
dc.identifier.citation
Martínez, Ana Laura; Quiroz, Facundo José; Carrera, Alicia Delia; Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing; King Saud University; Journal of the Saudi Society of Agricultural Sciences; 20; 6; 9-2021; 395-400
dc.identifier.issn
1658-077X
dc.identifier.uri
http://hdl.handle.net/11336/150651
dc.description.abstract
Plasmopara halstedii (Farl.) Berl. & De Toni causing downy mildew of sunflower is responsible for considerable economic losses worldwide. Because P. halstedii can be seed-transmitted, monitoring of seeds for pathogen contamination is important for the sunflower seed trade. The relevance of asymptomatic or latent infections as factors of disease spread have not been studied by molecular techniques. A molecular marker based on a putative effector gene of P. halstedii was used to examine the pathogen´s presence in asymptomatic sunflowers growing near patches of mildewed plants in naturally infected fields. The method based on conventional PCR was highly sensitive for detection of P. halstedii in DNA from whole seeds. By the application of this protocol, we found that all seed samples obtained from symptomatic plants amplified the expected fragment, whereas the diagnostic marker identified the presence of pathogen in one out of 21 asymptomatic plants. Possible uses of this marker to detect downy mildew in seed from asymptomatic plants or for race identification are discussed.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
King Saud University
dc.relation
https://doi.org/10.1016/j.jssas.2021.10.006
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
EFFECTOR MARKER
dc.subject
HELIANTHUS ANNUUS
dc.subject
PCR
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PLASMOPARA HALSTEDII
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SUNFLOWER DOWNY MILDEW
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SUNFLOWER SEEDS
dc.subject.classification
Agricultura
dc.subject.classification
Agricultura, Silvicultura y Pesca
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CIENCIAS AGRÍCOLAS
dc.title
Detection of Plasmopara halstedii in sunflower seeds: A case study using molecular testing
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-12-03T19:24:21Z
dc.journal.volume
20
dc.journal.number
6
dc.journal.pagination
395-400
dc.journal.pais
Arabia Saudita
dc.journal.ciudad
Riad
dc.description.fil
Fil: Martínez, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
dc.description.fil
Fil: Quiroz, Facundo José. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Balcarce; Argentina
dc.description.fil
Fil: Carrera, Alicia Delia. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
dc.journal.title
Journal of the Saudi Society of Agricultural Sciences
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jssas.2021.04.007
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1658077X21000503
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