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dc.contributor.author
Cardozo Gizzi, Andres Mauricio
dc.date.available
2022-01-11T16:55:42Z
dc.date.issued
2021-11
dc.identifier.citation
Cardozo Gizzi, Andres Mauricio; A Shift in Paradigms: Spatial Genomics Approaches to Reveal Single-Cell Principles of Genome Organization; Frontiers Media; Frontiers in Genetics; 12; 11-2021; 1-9
dc.identifier.uri
http://hdl.handle.net/11336/149934
dc.description.abstract
The genome tridimensional (3D) organization and its role towards the regulation of key cell processes such as transcription is currently a main question in biology. Interphase chromosomes are spatially segregated into “territories,” epigenetically-defined large domains of chromatin that interact to form “compartments” with common transcriptional status, and insulator-flanked domains called “topologically associating domains” (TADs). Moreover, chromatin organizes around nuclear structures such as lamina, speckles, or the nucleolus to acquire a higher-order genome organization. Due to recent technological advances, the different hierarchies are being solved. Particularly, advances in microscopy technologies are shedding light on the genome structure at multiple levels. Intriguingly, more and more reports point to high variability and stochasticity at the single-cell level. However, the functional consequences of such variability in genome conformation are still unsolved. Here, I will discuss the implication of the cell-to-cell heterogeneity at the different scales in the context of newly developed imaging approaches, particularly multiplexed Fluorescence in situ hybridization methods that enabled “chromatin tracing.” Extensions of these methods are now combining spatial information of dozens to thousands of genomic loci with the localization of nuclear features such as the nucleolus, nuclear speckles, or even histone modifications, creating the fast-moving field of “spatial genomics.” As our view of genome organization shifts the focus from ensemble to single-cell, new insights to fundamental questions begin to emerge.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
CHROMATIN 3D ARCHITECTURE
dc.subject
CHROMOSOME CONFORMATION
dc.subject
FLUORESCENCE IN SITU CELL HYBRIDIZATION (FISH)
dc.subject
GENOME ORGANIZATION
dc.subject
OLIGOPAINT
dc.subject
STOCHASTICITY
dc.subject
TOPOLOGICALLY ASSOCIATED DOMAIN (TAD)
dc.subject
TRANSCRIPTIONAL REGULATION
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
A Shift in Paradigms: Spatial Genomics Approaches to Reveal Single-Cell Principles of Genome Organization
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-01-06T14:51:24Z
dc.identifier.eissn
1664-8021
dc.journal.volume
12
dc.journal.pagination
1-9
dc.journal.pais
Suiza
dc.journal.ciudad
Lausana
dc.description.fil
Fil: Cardozo Gizzi, Andres Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Grupo Vinculado Centro de Investigación en Medicina Traslacional Severo R. Amuchástegui - Cimetsa | Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Grupo Vinculado Centro de Investigación en Medicina Traslacional Severo R. Amuchástegui - Cimetsa | Instituto de Investigación Médica Mercedes y Martín Ferreyra. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Grupo Vinculado Centro de Investigación en Medicina Traslacional Severo R. Amuchástegui - Cimetsa; Argentina. Instituto Universitario de Ciencias Biomédicas de Córdoba; Argentina
dc.journal.title
Frontiers in Genetics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fgene.2021.780822/full
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.3389/fgene.2021.780822
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