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Artículo

Cellular localization, cloning and expression of Leishmania braziliensis Phospholipase A1

Bott, EmanuelIcon ; López, María GabrielaIcon ; Lammel, Estela MaríaIcon ; Carfagna, Ivanna Emilce; Durante de Isola, Elvira Luisa; Ruybal, PaulaIcon ; Taboga, Oscar AlbertoIcon ; Gimenez, GuadalupeIcon ; Belaunzarán, María LauraIcon
Fecha de publicación: 04/2020
Editorial: Academic Press Ltd - Elsevier Science Ltd
Revista: Microbial Pathogenesis
ISSN: 0882-4010
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Leishmaniasis is caused by several species of protozoan parasites of the genus Leishmania and represents an important global health problem. Leishmania braziliensis in particular is responsible of cutaneous and mucocutaneous forms of this parasitosis, with prevalence in Latin America. In the present work, we describe in L. braziliensis promastigotes and amastigotes the presence of a Phospholipase A1 (PLA1) activity, an enzyme that catalyses extensive deacylation of phospholipids like phosphatidylcholine. In order to deepen the knowledge about L. braziliensis PLA1, the cloning and expression of the gene that codifies for this enzyme was carried out in a baculovirus expression system with the obtaintion of a purified recombinant protein that displayed PLA1 activity. Given that this is the first molecular and functional protein characterization of a PLA1 in the Leishmania genus, we also performed a phylogenetic analysis of this gene throughout 12 species whose genome sequences were available. The results presented here will contribute to increase the knowledge about trypanosome phospholipases, which could be novel and valuable as potential targets to fight neglected diseases like Leishmaniasis.
Palabras clave: BACULOVIRUS EXPRESSION SYSTEM , LEISHMANIA BRAZILIENSIS , PHOSPHATYDILCHOLINE , PHOSPHOLIPASE A1 , PHYLOGENETIC ANALYSIS
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/149696
URL: https://www.sciencedirect.com/science/article/abs/pii/S088240101931767
DOI: http://dx.doi.org/10.1016/j.micpath.2020.104010
Colecciones
Articulos(IMPAM)
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Citación
Bott, Emanuel; López, María Gabriela; Lammel, Estela María; Carfagna, Ivanna Emilce; Durante de Isola, Elvira Luisa; et al.; Cellular localization, cloning and expression of Leishmania braziliensis Phospholipase A1; Academic Press Ltd - Elsevier Science Ltd; Microbial Pathogenesis; 141; 4-2020; 1-11
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