Mostrar el registro sencillo del ítem
dc.contributor.author
Sukowati, Caecilia H. C.
dc.contributor.author
Anfuso, Beatrice
dc.contributor.author
Fiore, Esteban Juan
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Ie, Susan I.
dc.contributor.author
Raseni, Alan
dc.contributor.author
Vascotto, Fulvia
dc.contributor.author
Avellini, Claudio
dc.contributor.author
Mazzolini Rizzo, Guillermo Daniel
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Tiribelli, Claudio
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.date.available
2022-01-04T12:23:45Z
dc.date.issued
2019-03
dc.identifier.citation
Sukowati, Caecilia H. C.; Anfuso, Beatrice; Fiore, Esteban Juan; Ie, Susan I.; Raseni, Alan; et al.; Hyaluronic acid inhibition by 4-methylumbelliferone reduces the expression of cancer stem cells markers during hepatocarcinogenesis; Nature; Scientific Reports; 9; 1; 3-2019; 1-11
dc.identifier.issn
2045-2322
dc.identifier.uri
http://hdl.handle.net/11336/149548
dc.description.abstract
Hyaluronic acid (HA) is a glycosaminoglycan of extracellular matrix related to cell surface which interacts with various cell types. To understand the role of HA during hepatocarcinogenesis, we assessed the effect of the inhibition of HA deposition and its association with heterogeneous hepatocellular carcinoma (HCC) cells. In this study, we used transgenic mice C57BL/6J-Tg(Alb1HBV)44Bri/J (HBV-TG) and normal C57BL/6 J (WT) for in vivo study, while HCC cells Huh7 and JHH6 as in vitro models. Both models were treated with an HA inhibitor 4-methylumbelliferone (4MU). We observed that 4MU treatments in animal model down-regulated the mRNA expressions of HA-related genes Has3 and Hyal2 only in HBV-TG but not in normal WT. As observed in vivo, in HCC cell lines, the HAS2 mRNA expression was down-regulated in Huh7 while HAS3 in JHH6, both with or without the presence of extrinsic HA. Interestingly, in both models, the expressions of various cancer stem cells (CD44, CD90, CD133, and EpCAM) were also decreased. Further, histological analysis showed that 4MU treatment with dose 25 mg/kg/day reduced fibrosis, inflammation, and steatosis in vivo, in addition to be pro-apoptotic. We concluded that the inhibition of HA reduced the expressions of HA-related genes and stem cells markers in both models, indicating a possible modulation of cells-to-cells and cells-to-matrix interaction.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Nature
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
HCC
dc.subject
Cancer Stem Cells
dc.subject
Hyaluronic acid
dc.subject.classification
Otras Ciencias de la Salud
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Ciencias de la Salud
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
Hyaluronic acid inhibition by 4-methylumbelliferone reduces the expression of cancer stem cells markers during hepatocarcinogenesis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-11-20T18:10:41Z
dc.journal.volume
9
dc.journal.number
1
dc.journal.pagination
1-11
dc.journal.pais
Reino Unido
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.journal.ciudad
London
dc.description.fil
Fil: Sukowati, Caecilia H. C.. Università di Udine; Italia
dc.description.fil
Fil: Anfuso, Beatrice. No especifíca;
dc.description.fil
Fil: Fiore, Esteban Juan. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; Argentina
dc.description.fil
Fil: Ie, Susan I.. Eijkman Institute For Molecular Biology; Indonesia
dc.description.fil
Fil: Raseni, Alan. Institute for Research and Health Care Burlo Garofolo; Italia
dc.description.fil
Fil: Vascotto, Fulvia. Institute for Research and Health Care Burlo Garofolo; Italia
dc.description.fil
Fil: Avellini, Claudio. University Hospital Santa Maria della Misericordia; Italia
dc.description.fil
Fil: Mazzolini Rizzo, Guillermo Daniel. Universidad Austral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Tiribelli, Claudio. No especifíca;
dc.journal.title
Scientific Reports
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1038/s41598-019-40436-6
Archivos asociados