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dc.contributor.author
Pedroza Gómez, Yaretzi J.  
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Cossio Bayugar, Raquel  
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Aguilar Díaz, Hugo  
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Scarcella, Silvana Andrea  
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Reynaud, Enrique  
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del Rayo Sanchez Carbente, María  
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Narváez Padilla, Verónica  
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Miranda Miranda, Estefan  
dc.date.available
2022-01-03T13:04:43Z  
dc.date.issued
2021-11  
dc.identifier.citation
Pedroza Gómez, Yaretzi J.; Cossio Bayugar, Raquel; Aguilar Díaz, Hugo; Scarcella, Silvana Andrea; Reynaud, Enrique; et al.; Transcriptome-Based Identification of a Functional Fasciola hepatica Carboxylesterase B; Molecular Diversity Preservation International; Pathogens; 10; 11; 11-2021; 1-12  
dc.identifier.issn
2076-0817  
dc.identifier.uri
http://hdl.handle.net/11336/149487  
dc.description.abstract
Bioinformatics analysis of the complete transcriptome of Fasciola hepatica, identified a total of ten putative carboxylesterase transcripts, including a 3146 bp mRNA transcript coding a 2205 bp open reading frame that translates into a protein of 735 amino acids, resulting in a predicted protein mass of 83.5 kDa and a putative carboxylesterase B enzyme. The gene coding for this enzyme was found in two reported F. hepatica complete genomes stretching 23,230 bp, containing two exons of 1282 and 1864 bp, respectively, as well as a 20,084 bp intron between the exons. The enzymatic activity was experimentally assayed on F. hepatica protein extracts by SDS-PAGE zymograms using synthetic chromogenic substrates, confirming both the theoretical molecular weight and carboxylesterase enzymatic activity. Further bioinformatics predicted that this enzyme is an integral component of the cellular membrane that should be active as a 167 kDa homodimer complex and polyacrylamide gel electrophoresis (PAGE) zymograms experiments confirmed the analysis. Additional bioinformatics analysis showed that DNA sequences that code for this particular enzyme are highly conserved in other parasitic trematodes, although they are labeled hypothetical proteins.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Molecular Diversity Preservation International  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/  
dc.subject
BIOINFORMATICS  
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CARBOXYLESTERASE  
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FASCIOLOSIS  
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TRANSCRIPTOME  
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ZYMOGRAM  
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Otras Ciencias Veterinarias  
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Ciencias Veterinarias  
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CIENCIAS AGRÍCOLAS  
dc.title
Transcriptome-Based Identification of a Functional Fasciola hepatica Carboxylesterase B  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
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info:eu-repo/semantics/publishedVersion  
dc.date.updated
2021-12-13T18:48:08Z  
dc.journal.volume
10  
dc.journal.number
11  
dc.journal.pagination
1-12  
dc.journal.pais
Reino Unido  
dc.description.fil
Fil: Pedroza Gómez, Yaretzi J.. Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias; México  
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Fil: Cossio Bayugar, Raquel. Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias; México  
dc.description.fil
Fil: Aguilar Díaz, Hugo. Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias; México  
dc.description.fil
Fil: Scarcella, Silvana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina  
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Fil: Reynaud, Enrique. Universidad Nacional Autónoma de México; México  
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Fil: del Rayo Sanchez Carbente, María. Universidad Nacional Autónoma de México; México  
dc.description.fil
Fil: Narváez Padilla, Verónica. Universidad Nacional Autónoma de México; México  
dc.description.fil
Fil: Miranda Miranda, Estefan. Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias; México  
dc.journal.title
Pathogens  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/pathogens10111454  
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info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-0817/10/11/1454