Artículo
Loss of function of Arabidopsis NADP-malic enzyme 1 results in enhanced tolerance to aluminum stress
Badia, Mariana Beatriz
; Maurino, Verónica Graciela; Pavlovic, Tatiana
; Arias, Cintia Lucia
; Pagani, María Ayelén
; Andreo, Carlos Santiago
; Saigo, Mariana
; Drincovich, Maria Fabiana
; Gerrard Wheeler, Mariel Claudia
Fecha de publicación:
02/2020
Editorial:
Wiley Blackwell Publishing, Inc
Revista:
Plant Journal
ISSN:
0960-7412
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In acidic soils, aluminum (Al) toxicity is a significant limitation to crop production worldwide. Given its Al-binding capacity, malate allows internal as well as external detoxification strategies to cope with Al stress, but little is known about the metabolic processes involved in this response. Here, we analyzed the relevance of NADP-dependent malic enzyme (NADP-ME), which catalyzes the oxidative decarboxylation of malate, in Al tolerance. Plants lacking NADP-ME1 (nadp-me1) display reduced inhibition of root elongation along Al treatment compared with the wild type (wt). Moreover, wt roots exposed to Al show a drastic decrease in NADP-ME1 transcript levels. Although malate levels in seedlings and root exudates are similar in nadp-me1 and wt, a significant increase in intracellular malate is observed in roots of nadp-me1 after long exposure to Al. The nadp-me1 plants also show a lower H2O2 content in root apices treated with Al and no inhibition of root elongation when exposed to glutamate, an amino acid implicated in Al signaling. Proteomic studies showed several differentially expressed proteins involved in signal transduction, primary metabolism and protection against biotic and other abiotic stimuli and redox processes in nadp-me1, which may participate directly or indirectly in Al tolerance. The results indicate that NADP-ME1 is involved in adjusting the malate levels in the root apex, and its loss results in an increased content of this organic acid. Furthermore, the results suggest that NADP-ME1 affects signaling processes, such as the generation of reactive oxygen species and those that involve glutamate, which could lead to inhibition of root growth.
Palabras clave:
ALUMINUM TOXICITY
,
ARABIDOPSIS THALIANA
,
MALATE
,
ROOTS
,
STRESS SIGNALING
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Articulos(CEFOBI)
Articulos de CENTRO DE EST.FOTOSINTETICOS Y BIOQUIMICOS (I)
Articulos de CENTRO DE EST.FOTOSINTETICOS Y BIOQUIMICOS (I)
Citación
Badia, Mariana Beatriz; Maurino, Verónica Graciela; Pavlovic, Tatiana; Arias, Cintia Lucia; Pagani, María Ayelén; et al.; Loss of function of Arabidopsis NADP-malic enzyme 1 results in enhanced tolerance to aluminum stress; Wiley Blackwell Publishing, Inc; Plant Journal; 101; 3; 2-2020; 653-665
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