Artículo
The PhoP/PhoQ System and Its Role in Serratia marcescens Pathogenesis
Barchiesi, Julieta
; Castelli, Maria Eugenia
; Di Venanzio, Gisela Andrea
; Colombo, Maria Isabel
; Garcia Vescovi, Eleonora
Fecha de publicación:
06/2012
Editorial:
American Society for Microbiology
Revista:
Journal of Bacteriology
ISSN:
0021-9193
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Serratia marcescens is able to invade, persist, and multiply inside nonphagocytic cells, residing in nonacidic, nondegradative, autophagosome-like vacuoles. In this work, we have examined the physiological role of the PhoP/PhoQ system and its function in the control of critical virulence phenotypes in S. marcescens. We have demonstrated the involvement of the PhoP/PhoQ system in the adaptation of this bacterium to growth on scarce environmental Mg2+, at acidic pH, and in the presence of polymyxin B. We have also shown that these environmental conditions constitute signals that activate the PhoP/PhoQ system. We have found that the two S. marcescens mgtE orthologs present a conserved PhoP-binding motif and demonstrated that mgtE1 expression is PhoP dependent, reinforcing the importance of PhoP control in magnesium homeostasis. Finally, we have demonstrated that phoP expression is activated intracellularly and that a phoP mutant strain is defective in survival inside epithelial cells. We have shown that the Serratia PhoP/PhoQ system is involved in prevention of the delivery to degradative/acidic compartments.
Palabras clave:
PhoP/PhoQ
,
Serratia marcescens
,
Virulence
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Identificadores
Colecciones
Articulos(CEFOBI)
Articulos de CENTRO DE EST.FOTOSINTETICOS Y BIOQUIMICOS (I)
Articulos de CENTRO DE EST.FOTOSINTETICOS Y BIOQUIMICOS (I)
Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos(IHEM)
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Citación
Barchiesi, Julieta; Castelli, Maria Eugenia; Di Venanzio, Gisela Andrea; Colombo, Maria Isabel; Garcia Vescovi, Eleonora; The PhoP/PhoQ System and Its Role in Serratia marcescens Pathogenesis; American Society for Microbiology; Journal of Bacteriology; 194; 11; 6-2012; 2949-2961
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