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dc.contributor.author
Gonzalez Altamiranda, Erika Analia
dc.contributor.author
Arias, María E.
dc.contributor.author
Kaiser, Germán Gustavo
dc.contributor.author
Mucci, Nicolás Crescencio
dc.contributor.author
Odeón, Anselmo C.
dc.contributor.author
Felmer Dörner, Ricardo Nicolás
dc.date.available
2021-12-28T13:20:29Z
dc.date.issued
2020-11
dc.identifier.citation
Gonzalez Altamiranda, Erika Analia; Arias, María E.; Kaiser, Germán Gustavo; Mucci, Nicolás Crescencio; Odeón, Anselmo C.; et al.; Upregulation of interferon-alpha gene in bovine embryos produced in vitro in response to experimental infection with noncytophatic bovine-viral-diarrhea virus; Springer; Molecular Biology Reports; 47; 12; 11-2020; 9959-9965
dc.identifier.issn
0301-4851
dc.identifier.uri
http://hdl.handle.net/11336/149316
dc.description.abstract
In-vitro fertilization is a routine livestock-breeding technique widely used around the world. Several studies have reported the interaction of bovine viral-diarrhea virus (BVDV) with gametes and in-vitro-produced (IVP) bovine embryos. Since, gene expression in BVDV-infected IVP bovine embryos is scarcely addressed. The aim of this work was to evaluate the differential expression of genes involved in immune and inflammatory response. Groups of 20–25 embryos on Day 6 (morula stage) were exposed (infected) or not (control) to an NCP-BVDV strain in SOF medium. After 24 h, embryos that reached expanded blastocyst stage were washed. Total RNA of each embryo group was extracted to determine the transcription levels of 9 specific transcripts related with antiviral and inflammatory response by SYBR Green real time quantitative (RT-qPCR). Culture media and an aliquot of the last embryos wash on Day 7 were analyzed by titration and virus isolation, respectively. A conventional PCR confirmed BVDV presence in IVP embryos. A significantly higher expression of interferon-α was observed in blastocysts exposed to NCP-BVDV compared to the controls (p < 0.05). In this study, the upregulation of INFα and TLR7 genes involved in inflammatory and immune response in BVDV-infected IVP bovine embryos is a new finding in this field. This differential expression suggest that embryonic cells could function in a manner like immune cells by recognizing and responding early to interaction with viral pathogens. These results provide new insights into the action of BVDV on the complex molecular pathways controlling bovine early embryonic development.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BOVINE EMBRYOS
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BOVINE-VIRAL-DIARRHEA VIRUS
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GENE EXPRESSION
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INTERFERON-Α
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Otras Ciencias Veterinarias
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Ciencias Veterinarias
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CIENCIAS AGRÍCOLAS
dc.title
Upregulation of interferon-alpha gene in bovine embryos produced in vitro in response to experimental infection with noncytophatic bovine-viral-diarrhea virus
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-09-06T18:20:47Z
dc.journal.volume
47
dc.journal.number
12
dc.journal.pagination
9959-9965
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Gonzalez Altamiranda, Erika Analia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Buenos Aires Sur. Estacion Experimental Agropecuaria Balcarce. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible.; Argentina
dc.description.fil
Fil: Arias, María E.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
dc.description.fil
Fil: Kaiser, Germán Gustavo. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Buenos Aires Sur. Estacion Experimental Agropecuaria Balcarce. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible.; Argentina
dc.description.fil
Fil: Mucci, Nicolás Crescencio. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Buenos Aires Sur. Estacion Experimental Agropecuaria Balcarce. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible.; Argentina
dc.description.fil
Fil: Odeón, Anselmo C.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
dc.description.fil
Fil: Felmer Dörner, Ricardo Nicolás. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
dc.journal.title
Molecular Biology Reports
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s11033-020-05958-7
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s11033-020-05958-7
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