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dc.contributor.author
Shoda, L. K. M.
dc.contributor.author
Kegerreis, K. A.
dc.contributor.author
Suarez, C. E.
dc.contributor.author
Roditi, I.
dc.contributor.author
Corral, Ricardo Santiago
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dc.contributor.author
Bertot, Gustavo Miguel
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dc.contributor.author
Norimine, J.
dc.contributor.author
Brown, W. C.
dc.date.available
2021-12-20T20:48:34Z
dc.date.issued
2001-04
dc.identifier.citation
Shoda, L. K. M.; Kegerreis, K. A.; Suarez, C. E.; Roditi, I.; Corral, Ricardo Santiago; et al.; DNA from protozoan parasites Babesia bovis, Trypanosoma cruzi, and T. brucei is mitogenic for B lymphocytes and stimulates macrophage expression of interleukin-12, tumor necrosis factor alpha, and nitric oxide; American Society for Microbiology; Infection and Immunity; 69; 4; 4-2001; 2162-2171
dc.identifier.issn
0019-9567
dc.identifier.uri
http://hdl.handle.net/11336/149059
dc.description.abstract
The activation of innate immune responses by genomic DNA from bacteria and several nonvertebrate organisms represents a novel mechanism of pathogen recognition. We recently demonstrated the CpG-dependent mitogenic activity of DNA from the protozoan parasite Babesia bovis for bovine B lymphocytes (W. C. Brown, D. M. Estes, S. E. Chantler, K. A. Kegerreis, and C. E. Suarez, Infect. Immun. 66:5423-5432, 1998). However, activation of macrophages by DNA from protozoan parasites has not been demonstrated. The present study was therefore conducted to determine whether DNA from the protozan parasites B. bovis, Trypanosoma cruzi, and T. brucei activates macrophages to secrete inflammatory mediators associated with protective immunity. DNA from Escherichia coli and all three parasites stimulated B-lymphocyte proliferation and increased macrophage production of interleukin-12 (IL-12), tumor necrosis factor alpha (TNF-α), and nitric oxide (NO). Regulation of IL-12 and NO production occurred at the level of transcription. The amounts of IL-12, TNF-α, and NO induced by E. coli and protozoal DNA were strongly correlated (r2 > 0.9) with the frequency of CG dinucleotides in the genome, and immunostimulation by DNA occurred in the order E. coli ≥ T. cruzi > T. brucei > B. bovis. Induction of inflammatory mediators by E. coli, T. brucei, and B. bovis DNA was dependent on the presence of unmethylated CpG dinucleotides. However, at high concentrations, E. coli and T. cruzi DNA-mediated macrophage activation was not inhibited following methylation. The recognition of protozoal DNA by B lymphocytes and macrophages may provide an important innate defense mechanism to control parasite replication and promote persistent infection.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Microbiology
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
PROTOZOAN PARASITE DNA
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MACROPHAGE
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CYTOKINE EXPRESSION
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NITRIC OXIDE
dc.subject.classification
Inmunología
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
DNA from protozoan parasites Babesia bovis, Trypanosoma cruzi, and T. brucei is mitogenic for B lymphocytes and stimulates macrophage expression of interleukin-12, tumor necrosis factor alpha, and nitric oxide
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-12-20T17:25:04Z
dc.journal.volume
69
dc.journal.number
4
dc.journal.pagination
2162-2171
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Washington
dc.description.fil
Fil: Shoda, L. K. M.. Washington State University; Estados Unidos
dc.description.fil
Fil: Kegerreis, K. A.. Washington State University; Estados Unidos
dc.description.fil
Fil: Suarez, C. E.. Washington State University; Estados Unidos
dc.description.fil
Fil: Roditi, I.. Washington State University; Estados Unidos
dc.description.fil
Fil: Corral, Ricardo Santiago. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Bertot, Gustavo Miguel. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Laboratorio de Virología; Argentina
dc.description.fil
Fil: Norimine, J.. Washington State University; Estados Unidos
dc.description.fil
Fil: Brown, W. C.. Washington State University; Estados Unidos
dc.journal.title
Infection and Immunity
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1128/IAI.69.4.2162-2171.2001
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/IAI.69.4.2162-2171.2001
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC98143/
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