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Artículo

Structure and functional properties of the coldadapted catalase from Acinetobacter sp. Ver3 native to the Atacama plateau in northern Argentina

Sartorio, Mariana GabrielaIcon ; Cortez, Néstor Javier; Gonzalez, Javier MarceloIcon
Fecha de publicación: 03/2021
Editorial: International Union of Crystallography
Revista: Acta Crystallographica Section D: Structural Biology
ISSN: 2059-7983
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Heme catalases remove hydrogen peroxide by catalyzing its dismutation into water and molecular oxygen, thereby protecting the cell from oxidative damage. The Atacama plateau in northern Argentina, located 400014;m above sea level, is a desert area characterized by extreme UV radiation, high salinity and a large temperature variation between day and night. Here, the heme catalase KatE1 from an Atacama Acinetobacter sp. isolate was cloned, expressed and purified, with the aim of investigating its extremophilic properties. Kinetic and stability assays indicate that KatE1 is maximally active at 50°C in alkaline media, with a nearly unchanged specific activity between 0°C and 40°C in the pH range 5.5-11.0. In addition, its three-dimensional crystallographic structure was solved, revealing minimal structural differences compared with its mesophilic and thermophilic analogues, except for a conserved methionine residue on the distal heme side, which is proposed to comprise a molecular adaptation to oxidative damage.
Palabras clave: ACINETOBACTER , ATACAMA PLATEAU , CATALASES , COLD-ADAPTED ENZYMES
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/148304
URL: https://scripts.iucr.org/cgi-bin/paper?S2059798321000929
DOI: http://dx.doi.org/10.1107/S2059798321000929
Colecciones
Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos(INBIONATEC)
Articulos de INSTITUTO DE BIONANOTECNOLOGIA DEL NOA
Citación
Sartorio, Mariana Gabriela; Cortez, Néstor Javier; Gonzalez, Javier Marcelo; Structure and functional properties of the coldadapted catalase from Acinetobacter sp. Ver3 native to the Atacama plateau in northern Argentina; International Union of Crystallography; Acta Crystallographica Section D: Structural Biology; 77; 3-2021; 369-379
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