Artículo
Determination of benznidazole in human dried blood spots by liquid chromatography-mass spectrometry to monitor adherence to trypanosoma cruzi infection treatment in infants and children
Momper, Jeremiah D.; Hanan, Nathan J.; Rossi, Steven S.; Mirochnick, Mark H.; Cafferata, Maria Luisa; Lavenia, Antonia; Flores, Isolina; Gibbons, Luz; Ciganda, Alvaro; Sosa-Estani, Sergio Alejandro
; Capparelli, Edmund V.; Buekens, Pierre
Fecha de publicación:
07/2019
Editorial:
American Society of Tropical Medicine and Hygiene
Revista:
American Journal of Tropical Medicine and Hygiene
ISSN:
0002-9637
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Medication adherence is critical to the effectiveness of benznidazole (BZ) therapy for the treatment of Chagas disease. Assessing BZ adherence using traditional plasma sampling methods presents numerous challenges in resource-limited settings. Dried blood spot (DBS) sampling ofBZcan be used to overcome logistical barriers and provides a less invasive method for assessing BZ levels. ABZDBSassay using liquid chromatography-tandem mass spectrometry was developed and applied to a clinical study of infants and children being treated withBZ for Trypanosoma cruzi infection in Argentina. The assay was validated over a concentration range of 9.8-5,000 ng/mL. Inter-assay and intra-assay measures ranged from -2.9% to 2.7% and 0.5% to 8.3% for accuracy and from 3.5% to 12% and 1.6% to 13.6% for precision, respectively. The mean recovery of BZ was greater than 91%. Partitioning ratios for DBSs/plasma ranged from 0.95 to 1.02. A cohort of 10 infants and six children with T. cruzi infection being treated with BZ had median BZ concentrations of 1.2 (IQR 0.29, 2.14) μg/mL with seven of 65 (11%) samples above the BZ treatment goal of 3 μg/mL for adults. The reported DBS assay is a simple and accurate method for the quantitative measurement of BZ that can be applied to facilitate urgently needed clinical studies of BZfor the treatment of Chagas disease and assessBZadherence in resource-limited settings.
Palabras clave:
Chagas disease
,
Pharmacokinetic
,
Treatment
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Articulos(CIESP)
Articulos de CENTRO DE INVESTIGACIONES EN EPIDEMIOLOGIA Y SALUD PUBLICA
Articulos de CENTRO DE INVESTIGACIONES EN EPIDEMIOLOGIA Y SALUD PUBLICA
Citación
Momper, Jeremiah D.; Hanan, Nathan J.; Rossi, Steven S.; Mirochnick, Mark H.; Cafferata, Maria Luisa; et al.; Determination of benznidazole in human dried blood spots by liquid chromatography-mass spectrometry to monitor adherence to trypanosoma cruzi infection treatment in infants and children; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 101; 1; 7-2019; 116-122
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