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dc.contributor.author
Parodi, Adrián Rodrigo
dc.contributor.author
Asteasuain, Mariano
dc.contributor.author
Magario, Ivana
dc.date.available
2021-12-03T11:52:16Z
dc.date.issued
2021-12-10
dc.identifier.citation
Parodi, Adrián Rodrigo; Asteasuain, Mariano; Magario, Ivana; Kinetic analysis and evaluation of galactose oxidase activation by hematin in the green oxidation of glycerol; Elsevier Science SA; Biochemical Engineering Journal; 176; 10-12-2021; 1-11
dc.identifier.issn
1369-703X
dc.identifier.uri
http://hdl.handle.net/11336/148037
dc.description.abstract
Galactose oxidase (GAO) is the best-characterized member of a family of enzymes known as radical-copper oxidases, which use O2 as the final electron acceptor. GAO required an external oxidant to be fully activated and also an external scavenger of H2O2, the inhibiting product of the catalytic cycle. On the other hand, hematin (HEM) is a mimetic structure of heme peroxidases that also displays catalase-like activity. In this work, GAO was used in tandem with HEM towards glycerol (GLY) oxidation. Spectrometric and dissolved oxygen data are presented as evidence that the bi-catalytic system was active and stable. The optimized activity was 0.409 µmol h−1 UGAO−1, 5.2 times higher than with catalase. Optimum conditions were found at GAO 9.0 U/mL, GLY 1700 mM, HEM 108 µg/mL and a pH of 9.0. Kinetic modeling was used to propose a catalytic mechanism. The enzymatic constants KM and kb were obtained for GLY and galactose, the natural substrate of GAO. Moreover, the activation constant by HEM and the inhibition constant by H2O2 were estimated for GAO. Extrapolation of the model suggested a positive involvement of superoxide radicals in the activity of GAO, instead of triggering hematin inactivation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science SA
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BI-CATALYTIC SYSTEMS
dc.subject
DYNAMIC SIMULATION
dc.subject
GALACTOSE OXIDASE
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GLYCERALDEHYDE
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GLYCEROL OXIDATION
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KINETIC PARAMETERS
dc.subject.classification
Ingeniería Química
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Ingeniería Química
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
Kinetic analysis and evaluation of galactose oxidase activation by hematin in the green oxidation of glycerol
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-11-15T16:18:23Z
dc.journal.volume
176
dc.journal.pagination
1-11
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Parodi, Adrián Rodrigo. Universidad Nacional de Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada; Argentina
dc.description.fil
Fil: Asteasuain, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina. Universidad Nacional del Sur. Departamento de Ingeniería Química; Argentina
dc.description.fil
Fil: Magario, Ivana. Universidad Nacional de Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada; Argentina
dc.journal.title
Biochemical Engineering Journal
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1369703X21002795
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bej.2021.108203
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