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dc.contributor.author
Srednik, Mariela Elizabeth
dc.contributor.author
Grieben, Mario A.
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Bentancor, Adriana Beatriz
dc.contributor.author
Gentilini, Elida Raquel
dc.date.available
2021-12-03T11:30:12Z
dc.date.issued
2015-09
dc.identifier.citation
Srednik, Mariela Elizabeth; Grieben, Mario A.; Bentancor, Adriana Beatriz; Gentilini, Elida Raquel; Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of beta-lactam resistance; Journal of Infection in Developing Countries; Journal of Infection in Developing Countries; 9; 9; 9-2015; 1022-1027
dc.identifier.issn
2036-6590
dc.identifier.uri
http://hdl.handle.net/11336/148025
dc.description.abstract
Introduction: Bovine mastitis is a frequent cause of economic loss in dairy herds. Coagulase-negative staphylococci (CoNS) are increasing in importance as cause of bovine intramammary infection throughout the world in recent years. CoNS have been isolated from milk samples collected from cows with clinical and subclinical mastitis in several countries. Identification of mastitis pathogens is important when selecting appropriate antimicrobial therapy. Methodology: A total of 93 strains of Staphylococcus spp isolated from bovine mastitis in Argentina between 2010 and 2013 were identified by PCR-restriction fragment length polymorphism (PCR-RFLP) using the gap gene and were tested for the presence of blaZ and mecA genes by PCR and for the susceptibility to penicillin and cefoxitin by disk diffusion. Results: The most common CoNS species was S. chromogenes 46.2% (43/93), followed by S. devriesei 11.8% (11/93) and S. haemolyticus 9.7% (9/93). The blaZ gene was detected in 19 (20.4%), but only 16 (17.2%) isolates were resistant to penicillin; the mecA was detected in 6 (6.5%) isolates but only 4 (4.3) were resistant to cefoxitin. The 6 mecA-positive isolates showed oxacillin MICs = 0.5 µg/ml. Discussion: CoNS are important minor mastitis pathogens and can be the cause of substantial economic losses. The presence of methicillin resistant isolates emphasizes the importance of identification of CoNS when an intramammary infection is present because of the potential risk of lateral transfer of resistant genes among staphylococcal species.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Journal of Infection in Developing Countries
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ANTIMICROBIAL RESISTANCE
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BLAZ GENE
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BOVINE MASTITIS
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COAGULASE-NEGATIVE STAPHYLOCOCCI
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MECA GENE
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MOLECULAR IDENTIFICATION
dc.subject.classification
Otras Producción Animal y Lechería
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Producción Animal y Lechería
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CIENCIAS AGRÍCOLAS
dc.title
Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of beta-lactam resistance
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-10-27T20:12:02Z
dc.identifier.eissn
1972-2680
dc.journal.volume
9
dc.journal.number
9
dc.journal.pagination
1022-1027
dc.journal.pais
Italia
dc.description.fil
Fil: Srednik, Mariela Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
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Fil: Grieben, Mario A.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
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Fil: Bentancor, Adriana Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil
Fil: Gentilini, Elida Raquel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
dc.journal.title
Journal of Infection in Developing Countries
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3855/jidc.5871
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://jidc.org/index.php/journal
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