Artículo
Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina
Micheloud, Juan Francisco
; Aguirre Castro, Laura Sabrina
; Sandoval, Gabriela Virginia
; Avellaneda Caceres, Agustin
; Diodati, Julian Angel; Peralta, Andrea Verónica
Fecha de publicación:
29/07/2019
Editorial:
Springer
Revista:
Tropical Animal Health And Production
ISSN:
0049-4747
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Bovine papular stomatitis virus (BPSV) is a parapoxvirus associated with papular and erosive lesions on the muzzle, lips, and oral mucosa of cattle. BPSV infection occurs worldwide; however, it has still not been unequivocally diagnosed. The present report describes an outbreak of BPSV infection affecting dairy calves in northwestern Argentina and provides the first molecular characterization of this virus in the country. The disease was detected in a dairy farm, affecting 33 calves between 2 and 20 days of age. The signs included reddish papules, ulcers, and scabby proliferative lesions on muzzle, lips, and oral mucosa. The affected calves resisted to being fed due to severe local pain. Two necropsies were performed; papulas and ulcers were observed in ruminal and omasal mucosa. Histologically, the affected areas of the skin showed acanthosis, spongiosis, and parakeratotic hyperkeratosis with adjacent focally extensive ulcers and multifocal inflammatory infiltrate in the epidermis. Eosinophilic inclusion bodies were detected in the cytoplasm of epithelial cells. DNA extracted from scab samples was analyzed by PCR using pan-parapoxvirus primers for the B2L gene. The sequence analysis revealed 99%, 85%, and 84% similarity with BPSV, Pseudocowpox virus, and Orf virus, respectively. A phylogenetic tree constructed using the B2L sequence showed that the virus clustered with BPSV isolates. Although clinical cases compatible with BSPV infection have been frequently described in Argentina, the present report is the first to identify the agent associated with cattle disease in the country.
Palabras clave:
BOVINE PAPULAR STOMATITIS VIRUS
,
CATTLE
,
CUTANEOUS DISEASE
,
PARAPOXVIRUS
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos (IABIMO)
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Citación
Micheloud, Juan Francisco; Aguirre Castro, Laura Sabrina; Sandoval, Gabriela Virginia; Avellaneda Caceres, Agustin; Diodati, Julian Angel; et al.; Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina; Springer; Tropical Animal Health And Production; 52; 1; 29-7-2019; 453-459
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